首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠C-X-C趋化因子9(CXCL9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CXCL9含量。CXCL9是一个重要靶点。它属于趋化因子家族，在免疫调节等生理病理过程中发挥作用。其研究机制主要围绕与受体结合，招募免疫细胞，参与机体免疫反应，在肿瘤免疫、自身免疫病等领域是研究热点，有望为相关疾病治疗带来新策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。适用于感染性疾病模型、自身免疫疾病机制研究、肿瘤微环境分析等基础科研领域，为探索免疫细胞趋化、免疫调控网络及病理过程中CXCL9的动态变化提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cxcl9 ELISA Kit; Mig ELISA Kit; Scyb9C-X-C motif chemokine 9 ELISA Kit; Gamma-interferon-induced monokine ELISA Kit; Monokine induced by interferon-gamma ELISA Kit; MIG ELISA Kit; MuMIG ELISA Kit; Protein m119 ELISA Kit; Small-inducible cytokine B9 ELISA Kit
缩写：CXCL9
Uniprot No.：P18340
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 85 | |||||
| Range % | 82-89 | ||||||
| 1:2 | Average % | 106 | |||||
| Range % | 103-109 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
| 1:8 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
回收率：
| The recovery of mouse CXCL9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 93-99 | |||||
| EDTA plasma (n=4) | 102 | 98-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.4452.4012.4232.2875001.9381.9051.9221.7862501.2061.3011.2541.1181250.6670.6090.6380.50262.50.4050.3940.4000.26431.20.2560.2580.2570.12115.60.2090.2010.2050.06900.1370.1340.136 | |||||||
货期：3-5 working days
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