| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMA6 |
| Uniprot No | P60900 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-246aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMSRGSSAGFDRHITIFSPEGRLYQVE YAFKAINQGGLTSVAVRGKDCAVIVTQKKVPDKLLDSSTVTHLFKITENI GCVMTGMTADSRSQVQRARYEAANWKYKYGYEIPVDMLCKRIADISQVYT QNAEMRPLGCCMILIGIDEEQGPQVYKCDPAGYYCGFKATAAGVKQTEST SFLEKKVKKKFDWTFEQTVETAITCLSTVLSIDFKPSEIEVGVVTVENPK FRILTEAEIDAHLVALAERD |
| 预测分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSMA6重组蛋白的3篇参考文献及其摘要概括：
---
1. **文献名称**: "Expression and purification of recombinant human proteasome subunit alpha 6 (PSMA6) in *Escherichia coli*"
**作者**: Zhang Y, et al.
**摘要**: 研究报道了通过大肠杆菌系统成功表达并纯化人源PSMA6重组蛋白，优化了表达条件并通过质谱验证其正确性，为后续功能研究提供工具。
2. **文献名称**: "Structural analysis of the 20S proteasome subunit PSMA6 reveals its role in substrate recognition"
**作者**: Tanaka K, et al.
**摘要**: 通过X射线晶体学解析PSMA6重组蛋白的结构，揭示其在蛋白酶体复合体中对底物识别的作用，并探讨其与疾病相关突变的潜在关联。
3. **文献名称**: "Functional characterization of PSMA6 knockdown and recombinant protein rescue in cellular models"
**作者**: Wang L, et al.
**摘要**: 利用RNA干扰敲低细胞中PSMA6后，外源添加重组PSMA6蛋白可恢复蛋白酶体活性，证实其在维持蛋白质稳态中的关键功能。
---
注：以上文献信息为示例性质，实际文献需通过PubMed或SciHub等平台检索确认。若需具体文章，建议使用关键词“PSMA6 recombinant protein”或“proteasome subunit alpha 6 expression”进一步查询。
**Background of PSMA6 Recombinant Protein**
Proteasome 20S subunit alpha 6 (PSMA6) is a core component of the 20S proteasome complex, a critical player in the ubiquitin-proteasome system responsible for regulated intracellular protein degradation. This ATP-independent proteasome subunit facilitates the breakdown of misfolded, damaged, or redundant proteins into peptides, ensuring cellular homeostasis, protein quality control, and regulation of key processes like cell cycle progression, apoptosis, and immune response.
The PSMA6 gene encodes the α6 subunit, which contributes to the structural integrity of the 20S core. Recombinant PSMA6 protein is produced via genetic engineering, typically expressed in *E. coli* or mammalian systems, followed by purification to high homogeneity. This engineered protein retains the functional characteristics of native PSMA6. enabling researchers to study its biochemical interactions, structural roles, and regulatory mechanisms *in vitro*.
PSMA6 dysregulation has been implicated in pathologies such as cancers, neurodegenerative disorders (e.g., Parkinson’s and Alzheimer’s), and autoimmune diseases. Its recombinant form is widely used to investigate proteasome assembly, substrate specificity, and inhibitor screening for therapeutic development. Additionally, it serves as a tool to explore the molecular basis of diseases linked to proteasomal dysfunction. Recent studies also highlight its potential as a biomarker or target for drugs aiming to modulate proteasome activity, particularly in cancer therapy where proteasome inhibitors (e.g., bortezomib) are already clinically utilized.
Overall, PSMA6 recombinant protein provides a versatile platform for advancing both basic and translational research in proteostasis-related fields.
×
