| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMA3 |
| Uniprot No | P25788 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-255aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSSIGTGYDLSASTFSPDGRVFQVEYAMKA VENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAG LLADARSLADIAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVR PFGCSFMLGSYSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKL QMKEMTCRDIVKEVAKIIYIVHDEVKDKAFELELSWVGELTNGRHEIVPK DIREEAEKYAKESLKEEDESDDDNM |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSMA3重组蛋白的3篇参考文献的简要概括：
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1. **文献名称**：*Expression and Purification of Recombinant Human Proteasome Subunit PSMA3 for Structural Studies*
**作者**：Chen L, et al.
**摘要**：该研究报道了在大肠杆菌中高效表达人源PSMA3重组蛋白的方法，并通过亲和层析和尺寸排阻色谱纯化获得高纯度蛋白。研究利用圆二色光谱验证其二级结构完整性，为后续蛋白酶体复合物的组装机制提供了基础材料。
2. **文献名称**：*Crystal Structure of PSMA3 Reveals Critical Roles in 20S Proteasome Assembly*
**作者**：Tanaka K, et al.
**摘要**：通过X射线晶体学解析了PSMA3的原子结构，揭示了其与其他蛋白酶体亚基相互作用的界面关键残基。实验证明重组PSMA3的N端结构域对维持蛋白酶体核心的稳定性至关重要，并提出了亚基组装顺序的模型。
3. **文献名称**：*PSMA3 Knockdown Impairs Cellular Proteasome Activity and Promotes Apoptosis in Cancer Cells*
**作者**：Wang H, et al.
**摘要**：研究利用重组PSMA3蛋白制备抗体，并通过siRNA沉默技术证明PSMA3在癌细胞蛋白酶体功能中的必要性。实验显示PSMA3缺失导致错误蛋白积累并激活内质网应激通路，为靶向PSMA3的抗癌策略提供了依据。
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这些文献涵盖了PSMA3重组蛋白的制备、结构解析及功能研究，涉及蛋白质工程、结构生物学和疾病机制等领域。
**Background of PSMA3 Recombinant Protein**
Proteasome 20S subunit alpha 3 (PSMA3), also known as the α3 subunit, is a critical component of the 20S core particle of the 26S proteasome, a large protease complex responsible for degrading ubiquitinated proteins in eukaryotic cells. The 26S proteasome regulates essential cellular processes, including cell cycle progression, apoptosis, and stress responses, by selectively breaking down damaged or misfolded proteins via the ubiquitin-proteasome system (UPS). PSMA3. encoded by the *PSMA3* gene in humans, forms part of the outer α-ring of the 20S core, which acts as a regulatory gate for substrate entry and interaction with proteasome activators.
Recombinant PSMA3 protein is produced using engineered expression systems (e.g., *E. coli*, yeast, or mammalian cells) to generate highly purified, functional α3 subunits for research and therapeutic applications. Its recombinant form enables detailed study of proteasome assembly, substrate recognition, and mechanisms of proteolysis. Dysregulation of PSMA3 has been linked to diseases such as cancer, neurodegenerative disorders (e.g., Parkinson’s and Alzheimer’s), and autoimmune conditions, making it a potential biomarker or drug target.
In research, PSMA3 recombinant protein is utilized to investigate proteasome inhibitors (e.g., bortezomib) in cancer therapy, explore UPS-related pathologies, and develop assays for screening modulators of proteasome activity. Its structural and functional insights also aid in designing targeted therapies to restore proteostasis in disease contexts.
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