| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSEM3 |
| Uniprot No | P61289 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-254aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMASLLKVDQEVKLKVDSFRERITSEAEDLV ANFFPKKLLELDSFLKEPILNIHDLTQIHSDMNLPVPDPILLTNSHDGLD GPTYKKRRLDECEEAFQGTKVFVMPNGMLKSNQQLVDIIEKVKPEIRLLI EKCNTVKMWVQLLIPRIEDGNNFGVSIQEETVAELRTVESEAASYLDQIS RYYITRAKLVSKIAKYPHVEDYRRTVTEIDEKEYISLRLIISELRNQYVT LHDMILKNIEKIKRPRSSNAETLY |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设的示例性参考文献(请注意，PSEM3重组蛋白的相关研究可能较少，部分信息为演示用途，建议核实)：
1. **文献名称**: "Recombinant PSEM3 Protein Enhances Gamma-Secretase Activity in Alzheimer's Disease Models"
**作者**: Zhang, L. et al.
**摘要**: 研究通过大肠杆菌系统表达重组PSEM3蛋白，证实其可调控γ-分泌酶活性，促进Aβ肽段生成，为阿尔茨海默病机制提供新见解。
2. **文献名称**: "Purification and Functional Characterization of Human PSEM3 Recombinant Protein"
**作者**: Kim, S.J. & Patel, R.
**摘要**: 采用哺乳动物细胞表达系统纯化PSEM3蛋白，证明其与Notch信号通路相互作用，影响细胞分化过程。
3. **文献名称**: "Structural Insights into PSEM3 Recombinant Protein via Cryo-EM Analysis"
**作者**: Gupta, A. et al.
**摘要**: 通过冷冻电镜解析PSEM3的三维结构，揭示其跨膜结构域对底物识别的关键作用，为靶向药物设计提供依据。
4. **文献名称**: "PSEM3 Recombinant Expression in Insect Cells and Its Role in Cancer Metastasis"
**作者**: Chen, X. et al.
**摘要**: 在昆虫细胞中高效表达PSEM3.发现其过表达促进肿瘤细胞迁移，可能成为癌症治疗的潜在靶点。
**注意**：以上内容为示例，实际文献需通过PubMed/Google Scholar等平台以“PSEM3 recombinant”或相关关键词检索。若研究领域特殊，建议提供更多背景信息以便精准查询。
**Background of PSEM3 Recombinant Protein**
PSEM3 (Presenilin Enhancer Gamma-Secretase Subunit 3) is a recombinant protein engineered to study the molecular mechanisms underlying neurodegenerative diseases, particularly Alzheimer’s disease (AD). It functions as a critical component of the gamma-secretase complex, a multisubunit protease responsible for the cleavage of amyloid precursor protein (APP) to generate amyloid-beta (Aβ) peptides. Aberrant Aβ aggregation is a hallmark of AD, making gamma-secretase a key therapeutic target.
PSEM3 is derived from human presenilin, a catalytic core of the gamma-secretase complex. Recombinant PSEM3 is typically produced in mammalian expression systems (e.g., HEK293 cells) or *E. coli* via genetic engineering, ensuring high purity and activity. Its structure includes transmembrane domains essential for substrate recognition and enzymatic activity. Researchers utilize PSEM3 to dissect gamma-secretase’s role in APP processing, screen potential inhibitors, or investigate mutations linked to familial AD.
Beyond AD, PSEM3 has applications in studying Notch signaling, as gamma-secretase also processes Notch receptors, influencing cell differentiation and cancer pathways. The recombinant protein enables *in vitro* reconstitution of gamma-secretase activity, providing insights into substrate specificity and regulation.
Despite its utility, challenges remain in targeting gamma-secretase therapeutically due to its diverse substrate repertoire. PSEM3-based studies aim to develop selective modulators that reduce pathogenic Aβ without disrupting Notch signaling. Overall, PSEM3 serves as a vital tool for advancing molecular neuroscience and drug discovery in neurodegeneration.
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