| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRTFDC1 |
| Uniprot No | Q9NRG1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-225aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAGSSEEAPDYGRGVVIMDDWPGYDLN LFTYPQHYYGDLEYVLIPHGIIVDRIERLAKDIMKDIGYSDIMVLCVLKG GYKFCADLVEHLKNISRNSDRFVSMKVDFIRLKSYRNDQSMGEMQIIGGD DLSTLAGKNVLIVEDVVGTGRTMKALLSNIEKYKPNMIKVASLLVKRTSR SDGFRPDYAGFEIPNLFVVGYALDYNEYFRDLNHICVINEHGKEKYRV |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRTFDC1重组蛋白的3篇参考文献摘要(文献为虚构示例，仅作格式参考)：
1. **文献名称**: "Recombinant expression and functional characterization of human PRTFDC1 in purine metabolism"
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中重组表达了PRTFDC1蛋白，并证实其具有磷酸核糖基转移酶活性，参与调控细胞内嘌呤代谢途径，为研究其与遗传性代谢疾病的关联提供了基础。
2. **文献名称**: "Structural analysis of PRTFDC1 recombinant protein reveals novel substrate-binding domains"
**作者**: Chen L, et al.
**摘要**: 通过X射线晶体学解析了PRTFDC1重组蛋白的三维结构，发现其独特的底物结合域，并推测该结构可能影响其在肿瘤微环境中的异常代谢调控功能。
3. **文献名称**: "PRTFDC1 knockdown and recombinant protein rescue experiments highlight its role in neuronal development"
**作者**: Gupta R, et al.
**摘要**: 利用重组PRTFDC1蛋白进行功能回补实验，证明其在神经细胞分化中的必要性，并发现其通过调控嘌呤合成影响轴突生长和突触形成。
注：以上文献为示例，实际研究中建议通过PubMed或Web of Science检索真实文献。
PRTFDC1 (Phosphoribosyl Transferase Domain Containing 1) is a protein-coding gene implicated in cellular metabolism and disease pathogenesis. The gene encodes an enzyme homologous to hypoxanthine-guanine phosphoribosyltransferase (HGPRT), a key player in the purine salvage pathway responsible for recycling purine bases into nucleotides. However, unlike HGPRT, PRTFDC1 lacks catalytic activity due to amino acid substitutions in its active site, suggesting a distinct biological role. It is expressed in various tissues, including the brain, kidney, and liver, with elevated levels observed in certain cancers, such as renal cell carcinoma and breast cancer.
Recombinant PRTFDC1 protein is engineered for research applications to elucidate its physiological and pathological functions. Studies suggest PRTFDC1 may act as a "purine sensor," regulating nucleotide metabolism or interacting with signaling pathways like mTOR, though its exact mechanisms remain unclear. Its overexpression in tumors correlates with poor prognosis, potentially linking it to cancer cell proliferation or drug resistance. Additionally, PRTFDC1 mutations are associated with neurological disorders, including hereditary motor and sensory neuropathy, hinting at roles in neuronal maintenance.
The recombinant protein, typically produced in E. coli or mammalian expression systems, retains structural integrity for functional studies. It is utilized in enzyme activity assays, protein-protein interaction studies, and antibody development. Researchers also employ it to explore PRTFDC1’s role in metabolic reprogramming of cancer cells or its interaction with chemotherapeutic agents. Despite progress, the protein’s full biological significance and therapeutic potential warrant further investigation, particularly in the context of metabolic diseases and oncology.
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