| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRSS7 |
| Uniprot No | P98073 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 785-1019aa |
| 氨基酸序列 | MAIVGGSNAKEGAWPWVVGLYYGGRLLCGASLVSSDWLVSAAHCVYGRNL EPSKWTAILGLHMKSNLTSPQTVPRLIDEIVINPHYNRRRKDNDIAMMHL EFKVNYTDYIQPICLPEENQVFPPGRNCSIAGWGTVVYQGTTANILQEAD VPLLSNERCQQQMPEYNITENMICAGYEEGGIDSCQGDSGGPLMCQENNR WFLAGVTSFGYKCALPNRPGVYARVSRFTEWIQSFLH |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRSS7重组蛋白的3篇参考文献概览：
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1. **文献名称**: *"Cloning, Expression, and Characterization of Recombinant Human Enterokinase Light Chain"*
**作者**: LaVallie ER 等
**摘要**: 该研究报道了人PRSS7(肠激酶轻链)在大肠杆菌中的重组表达与纯化。作者通过优化表达条件获得高活性重组蛋白，并验证了其在切割融合蛋白标签中的应用，为蛋白质工程提供了高效工具。
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2. **文献名称**: *"Functional Analysis of Recombinant PRSS7 in Zebrafish Models of Digestive Disorders"*
**作者**: Zhang Y 等
**摘要**: 研究利用斑马鱼模型，探讨了重组PRSS7在肠道功能中的作用。结果表明，PRSS7通过激活胰蛋白酶原对消化功能至关重要，其缺失导致营养吸收障碍，提示其在肠道疾病治疗中的潜力。
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3. **文献名称**: *"Structural Insights into the Substrate Specificity of PRSS7: A Crystallographic Study"*
**作者**: Liu X 等
**摘要**: 通过X射线晶体学解析了重组PRSS7的三维结构，揭示了其底物识别位点的关键氨基酸残基。研究阐明了PRSS7对特定天冬氨酸序列(如DDDDK)的高效切割机制，为设计靶向抑制剂奠定基础。
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注：以上文献为示例性内容，实际引用时建议通过PubMed或Web of Science核对具体信息。如需进一步扩展，可补充针对PRSS7在癌症或代谢疾病中的研究文献。
PRSS7. also known as enteropeptidase or enterokinase, is a serine protease encoded by the *PRSS7* gene in humans. It is primarily synthesized in the duodenal mucosa and plays a critical role in digestive physiology by activating trypsinogen, the inactive precursor of the pancreatic protease trypsin. PRSS7 catalyzes the cleavage of trypsinogen into its active form, trypsin, which subsequently activates other digestive zymogens, initiating a proteolytic cascade essential for nutrient absorption. This unique substrate specificity—recognition of the DDDDK motif—makes PRSS7 indispensable for intestinal protein digestion.
Structurally, PRSS7 is a heterodimeric glycoprotein composed of a heavy chain and a light chain linked by disulfide bonds. The heavy chain mediates membrane anchoring and substrate recognition, while the light chain contains the catalytic domain with a conserved serine protease triad (His, Asp, Ser). Initially produced as an inactive zymogen (pro-enteropeptidase), PRSS7 undergoes autoactivation in the duodenum under acidic conditions, a process facilitated by bile salts.
Recombinant PRSS7 is engineered using expression systems like mammalian cells (e.g., CHO or HEK293) or insect cells to ensure proper post-translational modifications. Its recombinant form retains enzymatic specificity and is widely utilized in biotechnology and research. Applications include cleavage of fusion proteins in recombinant protein production, where PRSS7’s precision enables removal of affinity tags without damaging target proteins. Additionally, it serves as a tool to study protease-dependent pathways and digestive disorders linked to PRSS7 deficiencies, such as congenital enteropeptidase insufficiency, which causes malnutrition and growth defects. Research also explores its potential in therapeutic protein processing and diagnostic assays, underscoring its biomedical relevance.
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