| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PROSC |
| Uniprot No | O94903 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-275aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMWRAGSM SAELGVGCAL RAVNERVQQA VARRPRDLPA IQPRLVAVSK TKPADMVIEA YGHGQRTFGE NYVQELLEKA SNPKILSLCP EIKWHFIGHL QKQNVNKLMA VPNLFMLETV DSVKLADKVN SSWQRKGSPE RLKVMVQINT SGEESKHGLP PSETIAIVEH INAKCPNLEF VGLMTIGSFG HDLSQGPNPD FQLLLSLREE LCKKLNIPAD QVELSMGMSA DFQHAVEVGS TNVRIGSTIF GERDYSKKPT PDKCAADVKA PLEVAQEH |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PROSC重组蛋白的3-4篇参考文献的示例(注：PROSC相关文献可能较少，以下为模拟内容，建议通过学术数据库核实具体信息)：
---
1. **文献名称**：*"Expression and Purification of Recombinant PROSC Protein for Functional Studies"*
**作者**：Miller A, et al.
**摘要**：研究报道了在大肠杆菌中高效表达和纯化PROSC重组蛋白的方法，并通过体外实验验证了其作为磷酸酯酶辅助因子的活性，为后续功能研究奠定基础。
2. **文献名称**：*"Structural Insights into PROSC-Mediated Enzyme Activation"*
**作者**：Chen X, et al.
**摘要**：利用冷冻电镜和分子动力学模拟解析了PROSC重组蛋白的三维结构，揭示了其与靶酶结合的分子机制，阐明了其在催化反应中的调控作用。
3. **文献名称**：*"PROSC Recombinant Protein Enhances Metabolic Engineering Efficiency in Microbial Systems"*
**作者**：Lee S, et al.
**摘要**：研究将重组PROSC蛋白应用于微生物代谢工程，证明其通过优化辅因子循环显著提高目标产物的合成效率，具有工业生物技术应用潜力。
4. **文献名称**：*"Role of PROSC in Cellular Redox Homeostasis: Insights from Recombinant Protein Studies"*
**作者**：Patel R, et al.
**摘要**：通过体外重组PROSC蛋白实验，发现其参与调节细胞氧化还原平衡，可能成为治疗氧化应激相关疾病的潜在靶点。
---
**注意**：以上内容为模拟生成，PROSC可能指代特定研究中的蛋白(如磷酸酯酶相关蛋白)，建议通过PubMed、Google Scholar等平台以“PROSC recombinant protein”或“PROSC + 具体研究领域”为关键词检索最新文献。
PROSC recombinant proteins, often referred to in the context of protein engineering and biotechnology, are artificially engineered proteins produced through recombinant DNA technology. This process involves inserting a gene encoding the desired protein into a host organism (e.g., bacteria, yeast, or mammalian cells), which then expresses the protein in large quantities. The term "PROSC" may specifically denote a proprietary or specialized recombinant protein system, though it is occasionally used generically to describe high-purity, research-grade recombinant proteins designed for structural or functional studies.
The development of recombinant proteins revolutionized biomedical research and therapeutic applications. Traditional protein extraction from natural sources was limited by low yield, contamination risks, and ethical concerns. Recombinant technology addressed these challenges, enabling scalable production of proteins with defined sequences and modifications. PROSC-type proteins are typically optimized for stability, solubility, and functionality, making them vital tools in drug discovery, structural biology (e.g., X-ray crystallography, cryo-EM), and diagnostics. For instance, recombinant enzymes, antibodies, or cytokines produced via such systems are widely used in cancer therapy, vaccine development, and metabolic disorder treatments.
Advances in expression systems (e.g., Escherichia coli, CHO cells) and purification techniques (affinity chromatography, tag-based isolation) have enhanced the quality and specificity of these proteins. Challenges remain, including achieving proper post-translational modifications in non-mammalian hosts and minimizing aggregation. Emerging methods like cell-free synthesis and AI-driven protein design are pushing boundaries, allowing tailored proteins with novel functions. Overall, PROSC recombinant proteins exemplify the synergy between molecular biology and industrial innovation, underpinning breakthroughs in both basic science and clinical applications. Their continued refinement promises to accelerate personalized medicine and biomanufacturing efficiency.
×
