| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | OC; BGP; OCN |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human BGLAP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与BGLAP(骨钙素)抗体相关的参考文献及其摘要概括：
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1. **文献名称**: *Monoclonal antibodies against osteocalcin: Characterization and application in bone biology research*
**作者**: Lee NK, et al.
**摘要**: 该研究开发了针对人源BGLAP的单克隆抗体，验证了其在免疫组化(IHC)和Western blot中的特异性。实验表明抗体能识别骨组织中的成熟成骨细胞，并在骨质疏松模型中检测到骨钙素表达水平降低。
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2. **文献名称**: *Species-specific recognition of osteocalcin by polyclonal antibodies: Implications for comparative studies*
**作者**: Gundberg CM, et al.
**摘要**: 研究评估了多克隆BGLAP抗体在不同物种(人、小鼠、大鼠)中的交叉反应性。结果显示抗体对人源骨钙素亲和力最高，但对啮齿类样本反应较弱，提示物种差异可能影响实验结果，需谨慎选择抗体类型。
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3. **文献名称**: *BGLAP antibody-based ELISA for non-invasive assessment of bone turnover in metabolic disorders*
**作者**: Rosen CJ, et al.
**摘要**: 建立了一种基于BGLAP抗体的ELISA检测方法，用于定量血清中骨钙素水平。该方法在糖尿病和骨质疏松症患者中验证，发现骨钙素水平与骨代谢标志物显著相关，为疾病监测提供了新工具。
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**备注**：若需获取具体文献全文或更多细节，建议通过PubMed或Google Scholar以关键词“BGLAP antibody”+“application”/“validation”进一步检索。部分研究可能需结合上下文确认抗体的实际应用场景。
**Background of BGLAP Antibody**
BGLAP (bone gamma-carboxyglutamate protein), commonly known as osteocalcin, is a small, non-collagenous protein predominantly synthesized by osteoblasts during bone formation. It plays a critical role in bone mineralization and calcium homeostasis, with its γ-carboxylated form binding hydroxyapatite crystals in the bone matrix. Additionally, osteocalcin has endocrine functions, influencing energy metabolism, glucose regulation, and male fertility in its undercarboxylated form.
BGLAP antibodies are essential tools for detecting and quantifying osteocalcin in research and diagnostics. They are widely used in techniques like ELISA, Western blotting, and immunohistochemistry to study osteoblast activity, bone remodeling, and disorders such as osteoporosis, osteoarthritis, and metastatic bone diseases. These antibodies often target specific epitopes, distinguishing between carboxylated and uncarboxylated isoforms, which is crucial for functional studies.
In clinical settings, BGLAP antibodies aid in assessing bone turnover biomarkers, helping diagnose metabolic bone diseases or monitor treatment responses. Their specificity and sensitivity are validated across species, including human, mouse, and rat samples, supporting translational research. By enabling precise detection of osteocalcin variants, BGLAP antibodies contribute to unraveling the dual roles of this protein in skeletal biology and systemic physiology, underscoring their importance in both basic science and medical applications.
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