| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMB8 |
| Uniprot No | P28062 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 73-276aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMTTTLAFKFQHGVIAAVDSRASAGSY ISALRVNKVIEINPYLLGTMSGCAADCQYWERLLAKECRLYYLRNGERIS VSAASKLLSNMMCQYRGMGLSMGSMICGWDKKGPGLYYVDEHGTRLSGNM FSTGSGNTYAYGVMDSGYRPNLSPEEAYDLGRRAIAYATHRDSYSGGVVN MYHMKEDGWVKVESTDVSDLLHQYREANQ |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于PSMB8重组蛋白的简要参考文献示例(注：文献为虚构示例，实际引用时请核实真实来源)：
1. **文献名称**：**"Recombinant PSMB8 expression and its role in antigen processing"**
**作者**：Smith A, et al. (2015)
**摘要**：研究通过大肠杆菌系统成功表达并纯化重组人PSMB8蛋白，证明其在体外可增强蛋白酶体活性，促进MHC-I类分子抗原肽的加工。
2. **文献名称**：**"Structural analysis of recombinant PSMB8 in immunoproteasome assembly"**
**作者**：Tanaka K, et al. (2017)
**摘要**：通过X射线晶体学解析重组PSMB8蛋白结构，揭示其与PSMB9/PSMB10亚基的相互作用位点，为免疫蛋白酶体抑制剂设计提供依据。
3. **文献名称**：**"PSMB8 knockout and recombinant protein rescue in autoimmune disease models"**
**作者**：Zhang Y, et al. (2018)
**摘要**：在小鼠模型中验证重组PSMB8蛋白可逆转因基因缺失导致的自身免疫性肠炎表型，表明其在调节炎症反应中的关键作用。
4. **文献名称**：**"Recombinant PSMB8 modulates interferon-γ signaling in viral infection"**
**作者**：Lee S, et al. (2020)
**摘要**：研究发现重组PSMB8蛋白通过增强干扰素-γ信号通路，促进病毒抗原呈递，为抗病毒治疗提供新靶点。
(实际研究请参考PubMed、NCBI或专业期刊数据库获取真实文献。)
PSMB8. also known as β5i or LMP7. is a catalytic subunit of the immunoproteasome, a specialized form of the proteasome predominantly expressed in immune cells. Unlike the standard proteasome, the immunoproteasome is induced by inflammatory cytokines like IFN-γ and TNF-α, enhancing the production of antigenic peptides for MHC class I presentation. PSMB8 encodes the β5i subunit, which exhibits chymotrypsin-like activity critical for protein degradation and immune response regulation. Its role extends beyond antigen processing, influencing cytokine signaling, oxidative stress responses, and cell cycle control.
Mutations in the PSMB8 gene are linked to autoinflammatory disorders such as chronic atypical neutrophilic dermatosis with lipodystrophy and elevated temperatures (CANDLE syndrome), characterized by systemic inflammation and metabolic dysregulation. Dysregulated PSMB8 expression is also implicated in autoimmune diseases, neurodegenerative conditions, and cancer, where immunoproteasome activity may promote tumor cell survival or immune evasion.
Recombinant PSMB8 protein is produced in heterologous systems (e.g., E. coli or mammalian cells) for structural and functional studies. It enables researchers to investigate immunoproteasome assembly, substrate specificity, and inhibition mechanisms. Small-molecule inhibitors targeting PSMB8 are under exploration for therapeutic applications in autoimmune diseases and cancer. Recombinant PSMB8 also serves as an antigen for antibody development, aiding diagnostic assays and mechanistic studies of immunoproteasome-related pathologies. Its study provides insights into proteostasis in inflammation and offers potential avenues for modulating immune responses in disease contexts.
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