| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMB2 |
| Uniprot No | P49721 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-201aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMEYLIGIQGPDYVLVASDRVAASNIVQMKD DHDKMFKMSEKILLLCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAA ANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDY LAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKR FILNLPTFSVRIIDKNGIHDLDNISFPKQGS |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSMB2重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Expression and Purification of Recombinant Human Proteasome Subunit PSMB2 for Structural Studies"*
**作者**：Zhang Y, et al.
**摘要**：该研究报道了人源PSMB2重组蛋白在大肠杆菌中的高效表达与纯化方法，并通过X射线晶体学解析其三维结构，为蛋白酶体功能机制研究提供基础。
2. **文献名称**：*"Functional Characterization of PSMB2 in Cancer Cell Proliferation Using Recombinant Protein-Based Assays"*
**作者**：Lee S, et al.
**摘要**：通过重组PSMB2蛋白的体外功能实验，揭示其在癌细胞增殖中的调控作用，并证明靶向PSMB2可能抑制肿瘤生长，为癌症治疗提供潜在靶点。
3. **文献名称**：*"Development of a PSMB2-Specific Inhibitor Screening Platform Utilizing Recombinant Human Proteasome Subunits"*
**作者**：Wang H, et al.
**摘要**：研究构建了包含重组PSMB2蛋白的蛋白酶体活性检测体系，用于高通量筛选特异性抑制剂，为开发新型抗肿瘤药物提供技术基础。
(注：以上文献信息为模拟示例，实际引用需查询真实数据库。)
**Background of PSMB2 Recombinant Protein**
PSMB2 (Proteasome 20S Subunit Beta 2) is a critical component of the 20S proteasome, a multi-subunit protease complex responsible for the regulated degradation of intracellular proteins. As part of the ubiquitin-proteasome system, PSMB2 contributes to protein turnover, maintaining cellular homeostasis by breaking down damaged, misfolded, or redundant proteins. The 20S proteasome consists of α and β subunits arranged in four stacked rings (αββα). PSMB2. a β-type subunit, is essential for the proteolytic activity of the core particle, specifically participating in chymotrypsin-like activity, which cleaves peptide bonds after hydrophobic residues.
Recombinant PSMB2 protein is produced using genetic engineering techniques, often expressed in *E. coli* or mammalian systems, followed by purification to ensure high specificity and functionality. Its recombinant form enables detailed study of proteasome assembly, substrate processing, and regulatory mechanisms. Researchers utilize PSMB2 recombinant protein to investigate proteasome dysfunction linked to diseases such as cancer, neurodegenerative disorders (e.g., Alzheimer’s and Parkinson’s), and autoimmune conditions. It also serves as a tool for screening proteasome inhibitors, which are potential therapeutics for malignancies reliant on robust proteasome activity.
Additionally, PSMB2’s role in antigen processing for major histocompatibility complex (MHC) class I presentation highlights its relevance in immune regulation. Dysregulation of PSMB2 expression has been observed in various cancers, underscoring its potential as a biomarker or therapeutic target. Studies leveraging recombinant PSMB2 contribute to understanding proteasome biology and developing targeted therapies to modulate proteasomal activity in pathological states.
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