| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMA7 |
| Uniprot No | O14818 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-248aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MSYDRAITVF SPDGHLFQVE YAQEAVKKGS TAVGVRGRDI VVLGVEKKSV AKLQDERTVR KICALDDNVC MAFAGLTADA RIVINRARVE CQSHRLTVED PVTVEYITRY IASLKQRYTQ SNGRRPFGIS ALIVGFDFDG TPRLYQTDPS GTYHAWKANA IGRGAKSVRE FLEKNYTDEA IETDDLTIKL VIKALLEVVQ SGGKNIELAV MRRDQSLKIL NPEEIEKYVA EIEKEKEENE KKKQKKAS |
| 预测分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PSMA7重组蛋白的相关文献摘要信息(文献标题与内容为模拟示例，建议通过学术数据库核实)：
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1. **标题**：*"Recombinant PSMA7 expression and its role in 20S proteasome assembly"*
**作者**：H. Moriuchi et al.
**摘要**：研究利用大肠杆菌系统成功表达并纯化重组人PSMA7蛋白，通过体外重组实验证实PSMA7是20S蛋白酶体核心颗粒组装的关键亚基，其缺失导致蛋白酶体催化活性显著降低。
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2. **标题**：*"Structural insights into PSMA7 subunit in proteasome regulation"*
**作者**：C. P. Soares et al.
**摘要**：通过X射线晶体学解析了重组PSMA7的蛋白结构，揭示了其N端螺旋结构域在与其他蛋白酶体亚基互作中的关键作用，为靶向蛋白酶体的药物设计提供结构基础。
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3. **标题**：*"PSMA7 knockdown enhances cancer cell sensitivity to proteasome inhibitors"*
**作者**：C. D. Archer et al.
**摘要**：研究构建了重组PSMA7过表达/敲低细胞模型，发现PSMA7通过调控蛋白酶体活性影响多发性骨髓瘤细胞对Bortezomib的耐药性，提示其作为治疗靶点的潜力。
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4. **标题**：*"PSMA7 interacts with Parkinson's disease-related proteins in vitro"*
**作者**：L. Zhang et al.
**摘要**：利用重组PSMA7蛋白进行体外结合实验，发现其与α-synuclein和LRRK2存在直接相互作用，可能通过异常蛋白降解途径参与帕金森病的病理过程。
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建议通过PubMed或Web of Science以**"PSMA7 recombinant"**或**"proteasome subunit alpha 7 expression"**为关键词检索最新文献，重点关注功能机制或疾病关联研究。
**Background of PSMA7 Recombinant Protein**
PSMA7 (Proteasome 20S Subunit Alpha 7) is a critical component of the 20S core particle of the proteasome, a large protein complex responsible for the degradation of ubiquitinated proteins in eukaryotic cells. As part of the ubiquitin-proteasome system, PSMA7 plays a vital role in maintaining cellular homeostasis by regulating protein turnover, which impacts processes such as cell cycle progression, apoptosis, and stress response. Structurally, PSMA7 is an alpha-type subunit that forms the outer rings of the 20S proteasome, contributing to substrate recognition and gating access to the proteolytic core.
Recombinant PSMA7 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells*) to produce a purified, functional form of the subunit for experimental studies. This recombinant tool enables researchers to investigate proteasome assembly, enzymatic activity, and interactions with regulatory particles (e.g., 19S) or inhibitors. It is also utilized in drug discovery to screen compounds targeting proteasome activity, particularly in diseases like cancer and neurodegenerative disorders where proteasome dysfunction is implicated.
Studies involving PSMA7 recombinant protein have shed light on its post-translational modifications, such as phosphorylation, which may regulate proteasome function. Additionally, its overexpression or mutation has been linked to chemoresistance in cancers, highlighting its potential as a therapeutic target. The recombinant form is typically validated for purity (via SDS-PAGE), stability, and functional activity (e.g., peptide hydrolysis assays) to ensure reliability in biochemical and cell-based assays.
Overall, PSMA7 recombinant protein serves as a key reagent for dissecting proteasome biology and exploring strategies to modulate proteostasis in pathological conditions.
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