| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMB1 |
| Uniprot No | P20618 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-241aa |
| 氨基酸序列 | RFSPYVFNGGTILAIAGEDFAIVASDTRLSEGFSIHTRDSPKCYKLTDKTVIGCSGFHGDCLTLTKIIEARLKMYKHSNNKAMTTGAIAAMLSTILYSRRFFPYYVYNIIGGLDEEGKGAVYSFDPVGSYQRDSFKAGGSASAMLQPLLDNQVGFKNMQNVEHVPLSLDRAMRLVKDVFISAAERDVYTGDALRICIVTKEGIREETVSLRKD |
| 预测分子量 | 50.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSMB1重组蛋白的3篇示例参考文献，基于合理推测的典型研究方向整理：
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1. **文献名称**：**"Recombinant Expression and Functional Analysis of Human PSMB1 in a Bacterial System"**
**作者**：Chen L, Wang Y, et al.
**摘要**：本研究报道了在大肠杆菌中成功表达并纯化重组人PSMB1蛋白的优化方法。通过亲和层析和尺寸排阻色谱获得高纯度蛋白，并验证其在体外蛋白酶体复合体组装中的必要性。实验表明，重组PSMB1能恢复蛋白酶体的胰凝乳蛋白酶样活性，为研究其亚基特异性功能提供工具。
2. **文献名称**：**"Crystal Structure of PSMB1 Reveals Insights into Substrate Specificity of the Proteasome"**
**作者**：Tanaka K, et al.
**摘要**：本研究解析了重组PSMB1蛋白的晶体结构(分辨率2.8 Å)，揭示了其底物结合口袋的关键氨基酸残基。通过突变分析，发现PSMB1的活性位点构象影响蛋白酶体对多肽的切割效率，为设计特异性蛋白酶体抑制剂奠定结构基础。
3. **文献名称**：**"PSMB1 Knockdown and Recombinant Rescue Model Identifies Its Role in Cellular Oxidative Stress Response"**
**作者**：Guo R, et al.
**摘要**：通过siRNA敲低细胞中PSMB1表达，并利用重组PSMB1蛋白进行功能回补实验，发现PSMB1缺失导致活性氧(ROS)积累和细胞凋亡加剧。研究表明，PSMB1重组蛋白可恢复蛋白酶体降解氧化损伤蛋白的能力，提示其在氧化应激调控中的重要作用。
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**说明**：以上文献为示例性质，实际研究中需通过数据库(如PubMed、Web of Science)检索真实文献。建议使用关键词 **"PSMB1 recombinant"** 或 **"Proteasome subunit beta 1 expression"** 进行精准查询。
**Background of PSMB1 Recombinant Protein**
PSMB1 (Proteasome 20S Subunit Beta 1) is a critical component of the 20S proteasome, a multi-subunit protease complex responsible for the degradation of ubiquitinated proteins in eukaryotic cells. As part of the ubiquitin-proteasome system (UPS), PSMB1 contributes to the regulated turnover of intracellular proteins, impacting essential processes such as cell cycle progression, apoptosis, immune response, and stress adaptation. Structurally, the 20S proteasome comprises four stacked rings: two outer α-rings and two inner β-rings. PSMB1 is a β-type subunit, localized to the inner catalytic chamber, where its conserved threonine-dependent peptidase activity facilitates the cleavage of peptide bonds.
Recombinant PSMB1 protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian systems, to produce a purified, functional form for biochemical and cellular studies. Its recombinant production enables precise investigation of proteasome assembly, substrate specificity, and catalytic mechanisms. Researchers utilize PSMB1 recombinant proteins to study proteasome dysfunction linked to diseases, including neurodegenerative disorders (e.g., Alzheimer’s, Parkinson’s), autoimmune conditions, and cancers, where aberrant protein degradation pathways are implicated.
Additionally, recombinant PSMB1 serves as a tool for drug discovery, particularly in screening inhibitors or modulators targeting proteasome activity. Its structural and functional characterization aids in understanding resistance mechanisms to proteasome inhibitors (e.g., bortezomib) used in cancer therapy. Studies also explore PSMB1's role in antigen processing for MHC class I presentation, linking it to immune surveillance. By providing a controllable and scalable source of the subunit, recombinant PSMB1 advances both basic research and therapeutic development in proteostasis-related fields.
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