| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMA8 |
| Uniprot No | Q8TAA3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-250aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MASRYDRAIT VFSPDGHLFQ VEYAQEAVKK GSTAVGIRGT NIVVLGVEKK SVAKLQDERT VRKICALDDH VCMAFAGLTA DARVVINRAR VECQSHKLTV EDPVTVEYIT RFIATLKQKY TQSNGRRPFG ISALIVGFDD DGISRLYQTD PSGTYHAWKA NAIGRSAKTV REFLEKNYTE DAIASDSEAI KLAIKALLEV VQSGGKNIEL AIIRRNQPLK MFSAKEVELY VTEIEKEKEE AEKKKSKKSV |
| 预测分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSMA8重组蛋白的3篇代表性文献(虚拟示例，实际文献需根据具体研究检索)：
1. **文献名称**: "Recombinant PSMA8 Expression and Its Role in Spermatogenesis"
**作者**: Zhang L, et al.
**摘要**: 研究通过重组表达系统(如大肠杆菌)成功纯化PSMA8蛋白，发现其在哺乳动物精子发生过程中调控蛋白酶体活性，并参与减数分裂期蛋白质降解过程。
2. **文献名称**: "Structural Characterization of PSMA8 in the 20S Proteasome Complex"
**作者**: Tanaka K, et al.
**摘要**: 利用X射线晶体学解析重组PSMA8的结构，揭示其作为蛋白酶体α亚基的构象变化，并阐明其底物识别和催化机制在癌症相关蛋白降解中的作用。
3. **文献名称**: "PSMA8 Knockout Mice Reveal Embryonic Lethality and Immune Dysregulation"
**作者**: Wang Y, et al.
**摘要**: 通过构建PSMA8基因敲除小鼠模型，证明PSMA8缺失导致胚胎发育异常及免疫系统紊乱，提示其重组蛋白在治疗免疫相关疾病中的潜在价值。
---
如需真实文献，建议通过PubMed或Google Scholar检索关键词 "PSMA8 recombinant protein" 或 "proteasome alpha subunit 8"，筛选近年发表的实验性研究论文。
**Background of PSMA8 Recombinant Protein**
PSMA8 (Proteasome 20S Subunit Alpha 8), also known as PSMA7-like or α4-2. is a critical component of the 20S proteasome, a multisubunit protease complex responsible for the degradation of ubiquitinated proteins in the ubiquitin-proteasome system (UPS). This system is essential for maintaining cellular homeostasis by regulating protein turnover, quality control, and critical processes like cell cycle progression, apoptosis, and stress response.
The PSMA8 subunit belongs to the α-subunit family of the 20S core particle, which forms a barrel-shaped structure with β-subunits, creating catalytic chambers for proteolytic activity. Unlike constitutive α-subunits (e.g., PSMA1-7), PSMA8 is considered a tissue-specific or inducible variant, with elevated expression observed in immune cells, testes, and certain cancers. Its unique regulatory properties suggest specialized roles in modulating proteasome activity under specific physiological or pathological conditions.
Recombinant PSMA8 protein is produced using heterologous expression systems (e.g., *E. coli*, mammalian cells) to enable functional and structural studies. This engineered protein retains the ability to assemble into proteasomes, facilitating research on proteasome biogenesis, substrate specificity, and mechanisms of diseases linked to UPS dysregulation, such as neurodegenerative disorders (e.g., Alzheimer’s) and cancers. Additionally, recombinant PSMA8 serves as a tool for drug discovery, particularly in designing inhibitors or activators targeting proteasome activity.
Studies using PSMA8 recombinant protein have highlighted its potential role in immune responses, spermatogenesis, and cancer progression, making it a promising biomarker or therapeutic target. Its characterization continues to advance our understanding of proteasome diversity and adaptive cellular proteostasis.
×
