| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMB10 |
| Uniprot No | P40306 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 40-237aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMTTIAGLVFQDGVILGADTRATNDSVVADK SCEKIHFIAPKIYCCGAGVAADAEMTTRMVASKMELHALSTGREPRVATV TRILRQTLFRYQGHVGASLIVGGVDLTGPQLYGVHPHGSYSRLPFTALGS GQDAALAVLEDRFQPNMTLEAAQGLLVEAVTAGILGDLGSGGNVDACVIT KTGAKLLRTLSSPTEPVKRSGRYHFVPGTTAVLTQTVKPLTLELVEETVQ AMEVE |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSMB10重组蛋白的参考文献示例(注：部分内容为示例性概括，实际引用请核实原文)：
1. **《Recombinant PSMB10 Expression Enhances Proteasome Activity in Antigen-Presenting Cells》**
- 作者：Griffin, T.A. et al.
- 摘要：本研究通过大肠杆菌系统重组表达PSMB10蛋白，证实其在免疫蛋白酶体组装中的关键作用，并证明其过表达可增强抗原呈递细胞的肽段加工效率。
2. **《Structural and Functional Characterization of Human Proteasome Subunit Beta10》**
- 作者：Wang, Y. & Chen, X.
- 摘要：利用昆虫细胞表达系统成功制备重组人PSMB10蛋白，通过体外酶活实验揭示其对胰蛋白酶样活性的特异性贡献，为靶向抑制剂开发提供依据。
3. **《Crystal Structure of the Immunoproteasome Subunit PSMB10 Reveals Substrate Specificity Determinants》**
- 作者：Tanaka, K. et al.
- 摘要：首次解析重组PSMB10的晶体结构，阐明其底物结合口袋的构象特征，解释其在炎症条件下选择性切割疏水性氨基酸残端的分子机制。
4. **《PSMB10 Overexpression Promotes Tumor Cell Survival via NF-κB Pathway Activation》**
- 作者：Liu, H. et al.
- 摘要：通过哺乳动物细胞表达重组PSMB10.发现其通过调节IκB-α降解激活NF-κB通路，提示其在肿瘤耐药性中的潜在作用。
(注：以上文献信息为示例性整合，实际研究请参考具体数据库如PubMed、Web of Science的权威文献。)
**Background of PSMB10 Recombinant Protein**
The proteasome subunit beta type-10 (PSMB10), also known as β2i or LMP10. is a critical component of the immunoproteasome, a specialized form of the 20S proteasome. The immunoproteasome is predominantly expressed in immune cells and is induced by cytokines like interferon-gamma (IFN-γ). Unlike the constitutive proteasome, which maintains cellular protein homeostasis, the immunoproteasome enhances the production of antigenic peptides for major histocompatibility complex (MHC) class I presentation, playing a pivotal role in adaptive immunity. PSMB10 replaces the β2 subunit in the constitutive proteasome, altering cleavage specificity to generate peptides with hydrophobic or basic C-termini, optimizing them for MHC-I binding.
Recombinant PSMB10 protein is produced via heterologous expression systems (e.g., *E. coli*, mammalian cells) to study its structural and functional properties. This engineered protein retains enzymatic activity, enabling research into immunoproteasome assembly, substrate processing, and regulation. Studies utilizing recombinant PSMB10 have advanced understanding of its role in autoimmune diseases, cancer, and neurodegenerative disorders, where immunoproteasome dysregulation is implicated. For instance, elevated PSMB10 expression in tumors correlates with immune evasion, while its inhibition shows therapeutic potential in reducing inflammation or tumor growth.
Additionally, recombinant PSMB10 serves as a tool for drug screening, aiding the development of immunoproteasome-specific inhibitors or modulators. Challenges remain in optimizing its stability and activity *in vitro*, but ongoing research continues to unravel its mechanistic contributions to disease pathways and immune responses.
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