| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| WB Predicted band size | 123 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human DAAM1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DAAM1抗体的参考文献示例(注：以下为模拟内容，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*DAAM1 modulates actin dynamics via formin activity in cell migration*
**作者**：Wang X, et al.
**摘要**：本研究利用特异性DAAM1抗体，通过免疫荧光和Western blot技术，揭示了DAAM1通过Formin结构域调控肌动蛋白动态组装，促进癌细胞迁移的分子机制。
2. **文献名称**：*Development of a monoclonal antibody targeting DAAM1 for functional studies*
**作者**：Chen L, et al.
**摘要**：报道了一种小鼠源单克隆DAAM1抗体的开发与验证，通过ELISA和免疫组化证实其高特异性，并应用于斑马鱼模型研究DAAM1在胚胎发育中的作用。
3. **文献名称**：*DAAM1 mediates Wnt signaling in neuronal polarization*
**作者**：Kim S, et al.
**摘要**：结合DAAM1抗体进行共聚焦显微术，发现DAAM1通过响应Wnt信号调控神经元轴突的极性形成，依赖其与微管结合的能力。
4. **文献名称**：*DAAM1 antibody reveals distinct localization in cilia formation*
**作者**：Gomez G, et al.
**摘要**：通过定制DAAM1多克隆抗体，发现其在纤毛基体的定位，并证明DAAM1缺失导致纤毛结构异常，提示其在纤毛相关疾病中的潜在作用。
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**建议**：实际检索时可在PubMed或Google Scholar中使用关键词“DAAM1 antibody”或“DAAM1 immunolocalization”，筛选实验方法中明确使用该抗体的功能研究或抗体开发类文献。
DAAM1 (Dishevelled Associated Activator of Morphogenesis 1) is a formin-family protein involved in cytoskeletal reorganization and Wnt signaling pathways. It interacts with Dishevelled (Dvl) proteins to regulate actin dynamics, impacting cell polarity, migration, and tissue morphogenesis. DAAM1 is crucial in the planar cell polarity (PCP) pathway, a Wnt-mediated signaling cascade essential for embryonic development, organogenesis, and neural tube closure. It bridges membrane receptors and cytoskeletal effectors, facilitating actin polymerization and microtubule stabilization during processes like convergent extension in gastrulation.
DAAM1 antibodies are research tools designed to detect and quantify DAAM1 expression in cellular and tissue samples. They are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to study DAAM1's localization, interaction partners, and functional roles. These antibodies have been instrumental in linking DAAM1 dysregulation to pathological conditions, including cancer metastasis, neurodevelopmental disorders, and ciliopathies. For example, elevated DAAM1 expression correlates with invasive tumor behavior, while mutations are associated with neural tube defects. Researchers also employ DAAM1 antibodies to explore its crosstalk with other signaling pathways, such as Rho GTPase signaling, and its influence on stem cell differentiation. Available as polyclonal or monoclonal variants, these antibodies are typically validated in model organisms like mice, zebrafish, and human cell lines, aiding mechanistic studies in developmental biology and disease pathogenesis.
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