| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMD11 |
| Uniprot No | O00231 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-422aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSHMAAAAVVEFQRAQSLLSTDREASIDILHSIVKRDIQENDE EAVQVKEQSILELGSLLAKTGQAAELGGLLKYVRPFLNSISKAKAARLVR SLLDLFLDMEAATGQEVELCLECIEWAKSEKRTFLRQALEARLVSLYFDT KRYQEALHLGSQLLRELKKMDDKALLVEVQLLESKTYHALSNLPKARAAL TSARTTANAIYCPPKLQATLDMQSGIIHAAEEKDWKTAYSYFYEAFEGYD SIDSPKAITSLKYMLLCKIMLNTPEDVQALVSGKLALRYAGRQTEALKCV AQASKNRSLADFEKALTDYRAELRDDPIISTHLAKLYDNLLEQNLIRVIE PFSRVQIEHISSLIKLSKADVERKLSQMILDKKFHGILDQGEGVLIIFDE PPVDKTYEAALETIQNMSKVVDSLYNKAKKLT |
| 预测分子量 | 50 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSMD11重组蛋白的3篇代表性文献示例(注：部分内容基于领域知识模拟，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*Structural insights into the 26S proteasome regulatory subunit PSMD11*
**作者**：Lander GC, et al.
**摘要**：本研究通过冷冻电镜技术解析了人源26S蛋白酶体中PSMD11亚基的三维结构，揭示了其与邻近亚基PSMD9的相互作用界面，并探讨了PSMD11在蛋白酶体组装及底物识别中的潜在功能。
2. **文献名称**：*PSMD11 promotes tumor progression via modulating EGFR signaling in colorectal cancer*
**作者**：Wang Y, et al.
**摘要**：通过重组PSMD11蛋白体外功能实验发现，PSMD11通过稳定EGFR蛋白表达增强结直肠癌细胞增殖和迁移，抑制PSMD11可显著降低泛素-蛋白酶体介导的EGFR降解效率。
3. **文献名称**：*Optimization of recombinant PSMD11 expression in E. coli for structural studies*
**作者**：Zhang R, et al.
**摘要**：报道了一种高效表达和纯化重组PSMD11蛋白的大肠杆菌系统，通过密码子优化和温度诱导条件调控，获得高纯度蛋白用于后续结晶学和互作研究。
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**备注**：实际文献需通过PubMed、Web of Science等平台以“PSMD11”、“26S proteasome subunit”等关键词检索，重点关注重组蛋白表达、结构功能或疾病机制方向的研究。
PSMD11 (Proteasome 26S Subunit, Non-ATPase 11), also known as S9 or RPN6. is a critical component of the 26S proteasome, a large multi-subunit protease complex responsible for ubiquitin-dependent protein degradation in eukaryotic cells. As part of the 19S regulatory particle (RP) of the proteasome, PSMD11 plays a non-catalytic but essential role in recognizing ubiquitinated substrates, facilitating their deubiquitination, and directing them into the 20S catalytic core for proteolysis. This process is vital for maintaining cellular protein homeostasis, regulating cell cycle progression, apoptosis, and stress responses.
The PSMD11 gene is located on chromosome 19 in humans and encodes a 42-45 kDa protein containing multiple structural domains, including a conserved N-terminal PCI (Proteasome-COP9-eIF3) domain that mediates interactions with other proteasome subunits. Dysregulation of PSMD11 has been linked to various diseases, particularly cancers, where its overexpression correlates with tumor progression, chemoresistance, and poor prognosis. It has also been implicated in neurodegenerative disorders associated with protein aggregation.
Recombinant PSMD11 protein is typically produced using expression systems like E. coli or mammalian cells, enabling biochemical studies of proteasome assembly and function. Researchers utilize it to investigate substrate recognition mechanisms, screen for proteasome inhibitors, and study mutations affecting proteasome activity. As a research tool, it contributes to understanding the ubiquitin-proteasome system's role in disease pathogenesis and supports drug discovery efforts targeting proteasome dysfunction. Its recombinant form retains structural integrity for in vitro reconstitution of 19S regulatory particles and functional assays.
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