| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTGES2 |
| Uniprot No | A6NHH0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-206aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMKAVNEQGKEVTEFGNKYWLMLNEKEAQQV YGGKEARTEEMKWRQWADDWLVHLISPNVYRTPTEALASFDYIVREGKFG AVEGAVAKYMGAAAMYLISKRLKSRHRLQDNVREDLYEAADKWVAAVGKD RPFMGGQKPNLADLAVYGVLRVMEGLDAFDDLMQHTHIQPWYLRVERAIT EASPAH |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTGES2重组蛋白的模拟参考文献示例(实际文献需通过数据库核实)：
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1. **文献名称**: *"Characterization of recombinant human PTGES2: a prostaglandin E2 synthase involved in cellular stress responses"*
**作者**: Smith A, et al.
**摘要**: 研究报道了人源PTGES2重组蛋白在大肠杆菌中的高效表达与纯化，验证其作为膜结合型前列腺素E2合成酶的催化活性，并揭示其在氧化应激条件下调控PGE2合成的功能。
2. **文献名称**: *"Structural insights into PTGES2 catalysis and inhibition by small molecules"*
**作者**: Lee J, et al.
**摘要**: 通过X射线晶体学解析PTGES2重组蛋白的三维结构，阐明其底物结合位点及酶促反应机制，并筛选出特异性抑制剂，为抗炎药物开发提供依据。
3. **文献名称**: *"Functional crosstalk between PTGES2 and COX-2 in cancer-associated inflammation"*
**作者**: Zhang Y, et al.
**摘要**: 利用重组PTGES2蛋白验证其与COX-2的协同作用，证明两者在肿瘤微环境中共同促进PGE2生成，增强癌细胞增殖和免疫逃逸。
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注：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台以“PTGES2 recombinant protein”为关键词检索获取。
PTGES2 (Prostaglandin E Synthase 2) is a membrane-associated protein involved in the biosynthesis of prostaglandin E2 (PGE2), a critical lipid mediator regulating inflammation, pain perception, fever, and cellular homeostasis. Belonging to the glutathione-dependent microsomal prostaglandin E synthase family, PTGES2 catalyzes the isomerization of prostaglandin H2 (PGH2) to PGE2. acting downstream of cyclooxygenase (COX) enzymes. Unlike its inducible counterpart PTGES (also known as mPGES-1), PTGES2 is constitutively expressed in various tissues, including the brain, heart, and kidneys, suggesting broader physiological roles beyond inflammation.
Structurally, PTGES2 contains a glutathione-binding domain and associates with cellular membranes, particularly the endoplasmic reticulum and Golgi apparatus. Its activity is modulated by redox conditions and interacts with other proteins, such as COX-1 and COX-2. forming functional complexes for efficient PGE2 synthesis. Dysregulation of PTGES2 has been implicated in pathological conditions, including cancer, neurodegenerative diseases, and cardiovascular disorders, highlighting its potential as a therapeutic target.
Recombinant PTGES2 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable biochemical and pharmacological studies. This engineered protein retains enzymatic activity and is utilized to investigate PGE2 signaling pathways, screen inhibitors, and explore isoform-specific functions. Research on recombinant PTGES2 also aims to dissect its role in cellular stress responses, such as oxidative or endoplasmic reticulum stress, where it may contribute to cytoprotection or apoptosis. Despite progress, its regulatory mechanisms and tissue-specific functions remain incompletely understood, driving ongoing studies to clarify its contributions to health and disease.
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