| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMF1 |
| Uniprot No | Q92530 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-271aa |
| 氨基酸序列 | MAGLEVLFASAAPAITCRQDALVCFLHWEVVTHGYFGLGVGDQPGPNDKKSELLPAGWNNNKDLYVLRYEYKDGSRKLLVKAITVESSMILNVLEYGSQQVADLTLNLDDYIDAEHLGDFHRTYKNSEELRSRIVSGIITPIHEQWEKANVSSPHREFPPATAREVDPLRIPPHHPHTSRQPPWCDPLGPFVVGGEDLDPFGPRRGGMIVDPLRSGFPRALIDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPPGPNPDHLPPPGYDDMYL |
| 预测分子量 | 45.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSMF1重组蛋白的3篇参考文献示例(内容为虚构，供参考)：
---
1. **文献名称**：*Recombinant PSMF1 Expression in E. coli and Its Role in Proteasome Inhibition*
**作者**：Chen L., Wang Y., et al.
**摘要**：本研究成功构建了PSMF1重组蛋白的原核表达系统，利用大肠杆菌BL21(DE3)实现了高效可溶性表达，并通过镍柱亲和层析纯化获得高纯度蛋白。功能实验表明，重组PSMF1能够抑制20S蛋白酶体的活性，提示其作为内源性蛋白酶体抑制因子的潜在机制。
---
2. **文献名称**：*Structural Analysis of PSMF1 Reveals a Novel Chaperone-like Function*
**作者**：Smith J.R., Tanaka K., et al.
**摘要**：通过X射线晶体学解析了人源PSMF1重组蛋白的三维结构，发现其N端结构域含有独特的α-螺旋束，可能与结合蛋白酶体亚基有关。体外实验证实PSMF1通过构象变化促进蛋白酶体组装，为理解其分子伴侣功能提供了结构基础。
---
3. **文献名称**：*PSMF1 Knockdown Enhances Cellular Sensitivity to Oxidative Stress via Proteasome Dysregulation*
**作者**：Zhang H., Li M., et al.
**摘要**：利用重组PSMF1蛋白进行体外细胞实验，发现PSMF1缺失导致蛋白酶体活性异常升高，加剧活性氧(ROS)诱导的细胞凋亡。研究揭示了PSMF1在调控细胞氧化应激应答中的双重作用，可能为癌症治疗提供新靶点。
---
如需真实文献，建议通过PubMed或Google Scholar检索关键词“PSMF1 recombinant protein”或“PSMF1 proteasome”。
**Background of PSMF1 Recombinant Protein**
PSMF1 (Proteasome Inhibitor PI31 Subunit Family Member 1), also known as PI31. is a regulatory protein involved in modulating proteasome activity, a critical cellular machinery responsible for protein degradation. It binds to the 26S proteasome complex, influencing its assembly, localization, and function. PSMF1 is implicated in diverse cellular processes, including apoptosis, immune response, and protein quality control. Dysregulation of PSMF1 has been linked to neurodegenerative diseases, cancer, and autoimmune disorders, highlighting its importance in maintaining proteostasis and cellular health.
Recombinant PSMF1 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional forms of the protein for research. This allows scientists to study its structure, interactions, and mechanistic roles in vitro. The recombinant protein typically retains key functional domains, such as its proteasome-binding region, enabling investigations into how PSMF1 inhibits or stabilizes proteasome activity under varying conditions.
Studies utilizing PSMF1 recombinant protein have revealed its dual role: it can act as a proteasome inhibitor under stress or as a chaperone to facilitate proteasome assembly. Additionally, PSMF1 interacts with other proteins, such as immuno-responsive gene 1 (IRG1), suggesting broader regulatory networks. Its recombinant form is pivotal in drug discovery, particularly for diseases associated with proteasome dysfunction. By elucidating PSMF1’s molecular mechanisms, researchers aim to develop targeted therapies to restore proteostasis in pathological states.
Overall, PSMF1 recombinant protein serves as a vital tool for decoding proteasome regulation and advancing biomedical research into conditions linked to protein degradation imbalances.
×
