| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMD5 |
| Uniprot No | Q16401 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-504aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSEFMAAQALALLREVARLEAPLEELRAL HSVLQAVPLNELRQQAAELRLGPLFSLLNENHREKTTLCVSILERLLQAM EPVHVARNLRVDLQRGLIHPDDSVKILTLSQIGRIVENSDAVTEILNNAE LLKQIVYCIGGENLSVAKAAIKSLSRISLTQAGLEALFESNLLDDLKSVM KTNDIVRYRVYELIIEISSVSPESLNYCTTSGLVTQLLRELTGEDVLVRA TCIEMVTSLAYTHHGRQYLAQEGVIDQISNIIVGADSDPFSSFYLPGFVK FFGNLAVMDSPQQICERYPIFVEKVFEMIESQDPTMIGVAVDTVGILGSN VEGKQVLQKTGTRFERLLMRIGHQSKNAPVELKIRCLDAISSLLYLPPEQ QTDDLLRMTESWFSSLSRDPLELFRGISSQPFPELHCAALKVFTAIANQP WAQKLMFNSPGFVEYVVDRSVEHDKASKDAKYELVKALANSKTIAEIFGN PNYLRLRTYLSEGPYYVKPVSTTAVEGAE |
| 预测分子量 | 59 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSMD5重组蛋白的3篇参考文献及其摘要概括(文献信息为模拟示例，仅供参考)：
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1. **文献名称**：*Structural insights into the 19S regulatory particle of the proteasome: Role of PSMD5 in substrate recognition*
**作者**：Groll, M., et al. (2009)
**摘要**：该研究通过冷冻电镜和X射线晶体学解析了26S蛋白酶体中PSMD5的结构，揭示了其作为非ATP酶亚基在底物识别和去泛素化中的关键作用，为重组PSMD5的功能研究提供结构基础。
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2. **文献名称**：*PSMD5 overexpression promotes tumor progression via modulating NF-κB signaling in colorectal cancer*
**作者**：Hirano, T., et al. (2016)
**摘要**：本文发现PSMD5在结直肠癌中异常高表达，并通过重组蛋白实验证明其通过调控NF-κB通路促进肿瘤侵袭，提示PSMD5可能成为癌症治疗的潜在靶点。
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3. **文献名称**：*Recombinant PSMD5 production in E. coli: Optimization and functional characterization*
**作者**：Schmidt, C., et al. (2015)
**摘要**：研究优化了PSMD5重组蛋白在大肠杆菌中的表达和纯化流程，验证了其与蛋白酶体其他亚基的互作能力，为后续体外机制研究提供高效制备方法。
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**备注**：以上文献为示例，实际研究中建议通过PubMed或Google Scholar以“PSMD5 recombinant protein”或“PSMD5 proteasome”为关键词检索最新论文。
PSMD5 (Proteasome 26S Subunit, Non-ATPase 5) is a critical component of the 26S proteasome, a large multiprotein complex responsible for targeted protein degradation in eukaryotic cells. As part of the 19S regulatory particle, PSMD5 plays a regulatory role in the ubiquitin-proteasome system (UPS), which governs the controlled breakdown of ubiquitin-tagged proteins. This protein contains a conserved Jab1/MPN domain, suggesting potential deubiquitinating enzyme activity, though its exact catalytic mechanism remains under investigation.
Recombinant PSMD5 is engineered through molecular cloning, typically expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. Its production enables detailed study of proteasome assembly, substrate recognition, and regulatory mechanisms. Researchers utilize PSMD5 recombinant proteins to investigate its interactions with other proteasomal subunits (e.g., PSMC family ATPases) and ubiquitin receptors, shedding light on how the proteasome balances protein degradation with cellular homeostasis.
Dysregulation of PSMD5 has been implicated in cancer progression, neurodegenerative disorders (e.g., Parkinson's disease), and immune response modulation. Recombinant forms facilitate drug discovery efforts targeting proteasome dysfunction, particularly in developing inhibitors or stabilizers for therapeutic intervention. Current studies also explore its non-proteasomal roles, including potential involvement in DNA repair pathways and cell cycle regulation.
The availability of recombinant PSMD5 accelerates structural studies (e.g., cryo-EM analyses) and functional assays, providing insights into its role in maintaining protein quality control. Its biochemical characterization remains essential for understanding UPS-related pathologies and developing precision therapies.
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