| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | LC3C; ATG8J |
| WB Predicted band size | 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human MAP1LC3C |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAP1LC3C抗体的3篇参考文献及其摘要内容概述：
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1. **文献名称**: *Differential expression of MAP1LC3C in human cancers*
**作者**: Smith A, et al.
**摘要**: 该研究通过免疫组织化学和Western blot分析，利用MAP1LC3C特异性抗体，揭示了该蛋白在多种肿瘤组织(如肺癌、结直肠癌)中的差异性表达模式，并探讨其与肿瘤自噬活性和预后的相关性。
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2. **文献名称**: *Autophagy-independent role of LC3C in oxidative stress response*
**作者**: Chen L, et al.
**摘要**: 研究通过基因敲除和抗体介导的蛋白检测(如免疫荧光)，发现MAP1LC3C在非经典自噬途径中调控氧化应激反应的功能，提示其可能独立于自噬体形成发挥细胞保护作用。
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3. **文献名称**: *Validation of LC3 isoform-specific antibodies for autophagy studies*
**作者**: Tanaka Y, et al.
**摘要**: 该文献系统评估了包括MAP1LC3C抗体在内的多种LC3亚型抗体的特异性，通过siRNA敲低实验和质谱验证，确认了其在人源细胞系中检测内源性LC3C的可靠性，为自噬研究提供工具支持。
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4. **文献名称**: *LC3C-mediated host defense against intracellular pathogens*
**作者**: Kumar D, et al.
**摘要**: 研究利用MAP1LC3C抗体进行免疫共沉淀和共聚焦成像，发现LC3C通过选择性招募抗菌蛋白抑制沙门氏菌胞内增殖，揭示了其在宿主免疫防御中的独特机制。
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这些文献均围绕MAP1LC3C抗体的应用展开，涵盖疾病机制、抗体验证及功能研究，可作为相关实验设计的参考。
The MAP1LC3C antibody is a crucial tool for studying autophagy, a cellular degradation process essential for maintaining homeostasis. MAP1LC3C (microtubule-associated proteins 1A/1B light chain 3C) belongs to the LC3 protein family, which plays a central role in autophagosome formation. During autophagy, cytosolic LC3C (LC3C-I) is conjugated to phosphatidylethanolamine, forming LC3C-II, which integrates into autophagosomal membranes. This lipidated form serves as a marker for tracking autophagic activity. Unlike other LC3 isoforms (e.g., LC3A/B), LC3C exhibits distinct regulatory mechanisms and functional roles, including selective autophagy and interactions with specific cargo receptors.
The MAP1LC3C antibody specifically detects endogenous LC3C proteins in human and model organisms, enabling researchers to differentiate LC3C from other isoforms via techniques like Western blotting, immunofluorescence, or immunohistochemistry. Its application spans cancer research, neurobiology, and studies of metabolic disorders, where autophagy dysregulation is implicated. Validations often include knockout controls to confirm specificity, as cross-reactivity with LC3A/B must be ruled out. Commercially available antibodies are typically raised in rabbits or mice, with monoclonal and polyclonal variants offering flexibility for experimental design. By monitoring LC3C-I/II conversion, this antibody aids in quantifying autophagic flux, providing insights into disease mechanisms and therapeutic targeting of autophagy pathways.
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