| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | LARG; PRO2792 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ARHGEF12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ARHGEF12抗体的3篇参考文献概览：
1. **文献名称**：*ARHGEF12 regulates neuroblastoma cell migration via RhoA/ROCK signaling*
**作者**：Li Y et al.
**摘要**：研究利用ARHGEF12抗体通过Western blot和免疫荧光分析其在神经母细胞瘤细胞中的表达，发现ARHGEF12通过激活RhoA/ROCK通路调控细胞迁移，提示其在肿瘤转移中的作用。
2. **文献名称**：*The role of ARHGEF12 in cardiac hypertrophy*
**作者**：Wang H et al.
**摘要**：通过免疫共沉淀(Co-IP)结合ARHGEF12抗体，揭示其在心肌肥厚模型中与G蛋白偶联受体的相互作用，表明其通过调控Rho信号通路参与病理性心脏重构。
3. **文献名称**：*ARHGEF12 modulates dendritic spine formation in hippocampal neurons*
**作者**：Chen J et al.
**摘要**：研究使用ARHGEF12抗体进行免疫组化，发现其在大鼠海马神经元树突棘形态发生中的定位，证明其通过激活Cdc42影响突触可塑性，可能与神经系统疾病相关。
(注：以上文献信息为示例，实际文献需通过数据库检索获取。)
The ARHGEF12 antibody targets the ARHGEF12 protein, encoded by the ARHGEF12 gene (HGNC: 19123), which belongs to the Rho guanine nucleotide exchange factor (RhoGEF) family. This protein, also termed Lsc, activates Rho GTPases by facilitating the exchange of GDP for GTP, thereby regulating cytoskeletal dynamics, cell migration, and polarity. ARHGEF12 contains a tandem Dbl homology (DH) and pleckstrin homology (PH) domain critical for its GEF activity and membrane association. It interacts with heterotrimeric G proteins, linking G protein-coupled receptor (GPCR) signaling to Rho-mediated pathways.
ARHGEF12 antibodies are essential tools in studying its expression, localization, and function in cellular processes. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate ARHGEF12's role in physiological and pathological contexts. Dysregulation of ARHGEF12 has been implicated in cancers (e.g., hepatocellular carcinoma, glioblastoma) and neurological disorders, with studies suggesting its involvement in metastasis and neuronal development. Antibodies specific to ARHGEF12 help identify post-translational modifications, protein-protein interactions, and expression patterns across tissues. Commercial antibodies are typically validated for specificity using knockout controls or siRNA knockdown. Research utilizing these antibodies continues to elucidate ARHGEF12's contribution to disease mechanisms and potential therapeutic targets.
×
