| 纯度 | ＞80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTP1B |
| Uniprot No | P18031 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-435aa |
| 氨基酸序列 | MEMEKEFEQIDKSGSWAAIYQDIRHEASDFPCRVAKLPKNKNRNRYRDVS PFDHSRIKLHQEDNDYINASLIKMEEAQRSYILTQGPLPNTCGHFWEMVW EQKSRGVVMLNRVMEKGSLKCAQYWPQKEEKEMIFEDTNLKLTLISEDIK SYYTVRQLELENLTTQETREILHFHYTTWPDFGVPESPASFLNFLFKVRE SGSLSPEHGPVVVHCSAGIGRSGTFCLADTCLLLMDKRKDPSSVDIKKVL LEMRKFRMGLIQTADQLRFSYLAVIEGAKFIMGDSSVQDQWKELSHEDLE PPPEHIPPPPRPPKRILEPHNGKCREFFPNHQWVKEETQEDKDCPIKEEK GSPLNAAPYGIESMSQDTEVRSRVVGGSLRGAQAASPAKGEPSLPEKDED HALSYWKPFLVNMCVATVLTAGAYLCYRFLFNSNT |
| 预测分子量 | 71 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PTP1B重组蛋白的经典文献摘要概括：
1. **文献名称**：*Expression, Purification, and Characterization of the Human Catalytic Domain of Protein Tyrosine Phosphatase 1B*
**作者**：Zhang Z.Y. et al.
**摘要**：报道了人源PTP1B催化结构域的重组表达与纯化方法，通过大肠杆菌表达系统获得高活性蛋白，并分析其酶动力学特性，为后续抑制剂筛选奠定基础。
2. **文献名称**：*Crystal Structure of Human Protein Tyrosine Phosphatase 1B*
**作者**：Barford D. et al.
**摘要**：首次解析了人源PTP1B催化结构域的晶体结构，揭示了其底物结合口袋的三维构象，为基于结构的药物设计提供了关键依据。
3. **文献名称**：*Development of a High-Throughput Screening Assay for PTP1B Inhibitors Using Recombinant Protein*
**作者**：Combs A.P. et al.
**摘要**：利用重组PTP1B蛋白建立高通量抑制剂筛选平台，验证了多种天然化合物和小分子对其活性的抑制作用，为糖尿病治疗药物开发提供策略。
4. **文献名称**：*Allosteric Inhibition of PTP1B by a Novel Derivative Alters Obesity-Related Metabolic Pathways*
**作者**：Krishnan N. et al.
**摘要**：通过重组PTP1B蛋白研究其变构调节机制，发现新型化合物通过非催化位点调控酶活性，显著改善小鼠模型中的胰岛素抵抗和肥胖表型。
(注：以上文献信息为示例性概括，实际引用需核对原文准确性。)
**Background of PTP1B Recombinant Protein**
Protein tyrosine phosphatase 1B (PTP1B) is a key enzyme involved in the regulation of tyrosine phosphorylation-dependent signaling pathways. It belongs to the protein tyrosine phosphatase (PTP) superfamily and plays a critical role in modulating insulin and leptin signaling, as well as cell adhesion, proliferation, and immune responses. PTP1B is primarily localized to the endoplasmic reticulum (ER) membrane through a C-terminal hydrophobic segment but exerts its phosphatase activity in the cytosol by cleaving phosphotyrosine residues on target proteins.
Structurally, PTP1B consists of a catalytic domain containing the signature PTP motif (HCXXGXXR) essential for enzymatic activity and a regulatory domain that influences substrate specificity and cellular localization. Dysregulation of PTP1B activity has been linked to metabolic disorders such as type 2 diabetes, obesity, and certain cancers, making it a therapeutic target of interest.
Recombinant PTP1B protein is produced using expression systems like *E. coli* or mammalian cells, enabling large-scale purification for biochemical and structural studies. The recombinant form retains enzymatic activity, allowing researchers to investigate its substrate interactions, inhibition mechanisms, and role in disease pathways. Its production often involves affinity tags (e.g., GST or His-tag) for simplified purification and detection.
Studies utilizing PTP1B recombinant protein have advanced drug discovery efforts, particularly in developing small-molecule inhibitors to treat metabolic syndromes. Additionally, structural analyses (e.g., X-ray crystallography) of recombinant PTP1B have elucidated mechanisms of catalysis and regulation, providing insights into its allosteric modulation and redox-sensitive inactivation. Overall, PTP1B recombinant protein serves as a vital tool for understanding tyrosine phosphatase biology and exploring therapeutic interventions.
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