| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | PHKG |
| WB Predicted band size | 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PHKG1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PHKG1抗体的3篇参考文献示例(注：以下信息为模拟生成，实际文献需通过学术数据库验证)：
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1. **文献名称**：*PHKG1 regulates hepatic glycogen metabolism and protects against steatosis in mice*
**作者**：Smith A, et al.
**摘要**：研究利用PHKG1特异性抗体检测小鼠肝脏中PHKG1蛋白表达，发现PHKG1通过调控糖原磷酸化酶活性影响糖原分解，其缺失导致肝脂肪变性，提示PHKG1在代谢疾病中的潜在作用。
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2. **文献名称**：*Development and validation of a novel monoclonal antibody for PHKG1 in human skeletal muscle*
**作者**：Chen L, et al.
**摘要**：报道一种新型PHKG1单克隆抗体的开发，通过Western blot和免疫组化验证其特异性，应用于人类骨骼肌样本，揭示PHKG1在肌肉萎缩症患者中的表达下调。
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3. **文献名称**：*PHKG1-mediated autophagy suppression promotes hepatocellular carcinoma progression*
**作者**：Wang Y, et al.
**摘要**：利用PHKG1抗体进行免疫沉淀和免疫荧光实验，发现PHKG1通过抑制自噬途径促进肝癌细胞增殖，为PHKG1作为肝癌治疗靶点提供依据。
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**注意事项**：
- 实际文献需通过PubMed、Google Scholar等平台以关键词“PHKG1 antibody”或“PHKG1 expression”检索。
- 建议关注涉及PHKG1抗体在疾病模型(如癌症、代谢疾病)、信号通路(如糖原代谢、自噬)或抗体开发验证的研究。
**Background of PHKG1 Antibody**
The PHKG1 (phosphorylase kinase gamma 1) antibody is a tool used to detect and study the PHKG1 protein, a catalytic subunit of the phosphorylase kinase (PhK) complex. This complex plays a critical role in glycogen metabolism by activating glycogen phosphorylase, which initiates glycogen breakdown into glucose-1-phosphate for energy production. PHKG1 is predominantly expressed in the liver and skeletal muscle, reflecting its role in regulating energy homeostasis. Structurally, PHKG1 contains a conserved kinase domain essential for its enzymatic activity.
Research involving PHKG1 antibodies has focused on understanding its physiological and pathological roles. Studies link PHKG1 dysfunction to glycogen storage diseases (GSDs) and metabolic disorders. Recently, PHKG1 has also been implicated in cancer biology, with altered expression observed in hepatocellular carcinoma (HCC) and other malignancies, suggesting potential roles in tumor progression or metabolic reprogramming.
PHKG1 antibodies are widely utilized in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and interactions. Validated antibodies help explore PHKG1’s regulatory mechanisms, including its phosphorylation-dependent activity and crosstalk with signaling pathways like AMPK or insulin signaling.
Overall, PHKG1 antibodies serve as vital reagents for dissecting glycogen metabolism, metabolic diseases, and cancer, offering insights into therapeutic targeting opportunities.
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