首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人脱氢表雄酮(DHEA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的DHEA含量。DHEA即脱氢表雄酮，是体内肾上腺皮质分泌的一种类固醇激素。它在人体代谢、免疫等方面有重要作用。研究机制主要围绕其对神经、生殖等系统的调节，如改善认知功能、影响性激素水平等，相关研究有望为多种疾病防治提供新思路。试剂盒检测范围为0.1 ng/mL-20 ng/mL，适用于内分泌代谢研究、生殖系统功能评估、衰老机制探索等科研领域，特别适用于激素水平动态监测、药物干预效果评价等实验场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：DHEA
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.1 ng/mL-20 ng/mL
灵敏度：0.05 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DHEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-110 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 87-100 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-104 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-100 | |||||
回收率：
| The recovery of human DHEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-102 | ||||
| EDTA plasma (n=4) | 96 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average200.1950.1990.19750.3590.3420.3511.60.6100.6240.6170.41.0081.0231.0160.11.4281.4351.43202.4392.4422.441 | ||||||
货期：3-5 working days
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