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产品描述：人脱氢表雄酮硫酸酯(DHEA-S)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DHEA-S含量。DHEA - S即硫酸脱氢表雄酮，是肾上腺分泌的一种类固醇激素。它在人体代谢、免疫等生理过程中发挥作用。研究机制上，其参与多种信号通路，与激素平衡、细胞增殖等密切相关，在衰老、代谢疾病等领域是研究热点。试剂盒检测范围为20 ng/mL-4000 ng/mL，适用于基础研究领域中对激素代谢机制、内分泌系统功能评估、药物干预效果分析等科研项目，为探索肾上腺功能、衰老相关激素变化或代谢性疾病模型提供可靠的数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：DHEA-S
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：20 ng/mL-4000 ng/mL
灵敏度：10 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DHEA-S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-103 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 89-105 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 90-100 | ||||||
| 1:8 | Average % | 90 | |||||
| Range % | 83-110 | ||||||
回收率：
| The recovery of human DHEA-S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 86-103 | |||||
| EDTA plasma (n=4) | 89 | 84-93 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average01.9531.9171.935201.3211.3391.330800.8430.8550.8493200.5110.5300.52110000.3290.3240.32740000.1890.1840.187 | |||||||
货期：3-5 working days
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