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产品描述：牛脱氧核糖核酸酶1(DNASE1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DNASE1含量。DNASE1是重要的靶点。它是一种核酸酶，可降解DNA。背景上，其在维持细胞内环境稳定等方面有重要意义。研究机制方面，主要聚焦其降解DNA的具体过程、调控因素，以及在相关疾病如自身免疫病中因功能异常对病情发展的影响。试剂盒检测范围为12.5 pg/mL-800 pg/mL，可为科研领域研究牛DNASE1在细胞凋亡机制、炎症反应或免疫调节中的功能提供可靠工具，尤其适用于畜牧病理学、动物免疫学研究中对相关生物标志物的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DNASE1 ELISA Kit; DNL1Deoxyribonuclease-1 ELISA Kit; EC 3.1.21.1 ELISA Kit; Deoxyribonuclease I ELISA Kit; DNase I ELISA Kit
缩写：DNASE1
Uniprot No.：P00639
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.1 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine DNASE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:20 | Average % | 85 | |||||
| Range % | 80-89 | ||||||
| 1:40 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
| 1:80 | Average % | 103 | |||||
| Range % | 99-109 | ||||||
| 1:160 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
回收率：
| The recovery of bovine DNASE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 88 | 84-92 | |||||
| EDTA plasma (n=4) | 93 | 89-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected8002.3992.4162.4082.2734002.2132.1952.2042.0692001.8131.7411.7771.6421001.2041.2271.2161.081500.6830.7130.6980.563250.4000.3920.3960.26112.50.2920.2840.2880.15300.1310.1380.135 | |||||||
货期：3-5 working days
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