Human Mitochondrial import inner membrane translocase subunit TIM14(DNAJC19) ELISA kit

产品详情

中文名： 人线粒体进口内膜转位酶亚基TIM14(DNAJC19)酶联免疫试剂盒

货号: IPD-EL007026HU

Price： ￥2500

48T 96T

数量：

大包装询价

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：人线粒体进口内膜转位酶亚基TIM14(DNAJC19)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的DNAJC19含量。DNAJC19是一种基因，作为治疗非小细胞肺癌的靶点。研究发现，该基因参与NSCLC细胞的增殖和迁移，通过抑制其表达可以有效降低肿瘤细胞的侵袭、迁移和生长能力，成为NSCLC患者的潜在治疗靶标。试剂盒检测范围为28 pg/mL-1800 pg/mL，通过高特异性抗体对靶蛋白进行精准捕获与信号放大，可为线粒体蛋白质转运机制研究、疾病模型中线粒体功能障碍评估以及药物干预效果分析提供可靠工具，广泛应用于神经退行性疾病、心血管疾病等科研领域的分子机制探索及生物标志物定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DnaJ (Hsp40) homolog; subfamily C; member 19 ELISA Kit; DnaJ homolog subfamily C member 19 ELISA Kit; DNAJC19 ELISA Kit; homolog of yeast TIM14 ELISA Kit; Mitochondrial import inner membrane translocase subunit TIM14 ELISA Kit; PAM18 ELISA Kit; TIM 14 ELISA Kit; TIM14 ELISA Kit; TIM14_HUMAN ELISA Kit; TIMM 14 ELISA Kit; TIMM14 ELISA Kit; translocase of the inner mitochondrial membrane 14 ELISA Kit; Translocase of the inner mitochondrial membrane 14; yeast homolog of ELISA Kit
缩写：DNAJC19
Uniprot No.：Q96DA6
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：28 pg/mL-1800 pg/mL
灵敏度：7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DNAJC19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	86
	Range %	81-90
1:4	Average %	93
	Range %	89-98
1:8	Average %	90
	Range %	85-94

回收率：

The recovery of human DNAJC19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-107
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/mlOD1OD2AverageCorrected18002.6562.7192.6882.5799002.2872.1722.2302.1214501.6531.7071.6801.5712251.1351.0711.1030.994112.50.6810.6530.6670.558560.3740.3620.3680.259280.2570.2520.2550.14600.1120.1050.109