| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | STL2; COLL6; CO11A1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human COL11A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于RNF5抗体的参考文献及其摘要概括：
1. **文献名称**：*RNF5-mediated ubiquitination regulates the degradation of mutant p53 in cancer cells*
**作者**：Li, Z., et al.
**摘要**：该研究利用RNF5特异性抗体，发现RNF5通过泛素化修饰促进突变型p53蛋白的降解，抑制肿瘤细胞的增殖和转移，揭示了RNF5在癌症治疗中的潜在靶点作用。
2. **文献名称**：*RNF5 modulates immune checkpoint PD-L1 through endoplasmic reticulum-associated degradation*
**作者**：Jiang, Y., et al.
**摘要**：通过免疫沉淀(IP)结合RNF5抗体，研究发现RNF5调控PD-L1的内质网相关降解(ERAD)，影响肿瘤免疫逃逸，为免疫治疗耐药性提供了新机制解释。
3. **文献名称**：*RNF5-dependent regulation of mitochondrial dynamics and apoptosis in neurodegenerative models*
**作者**：Chen, X., et al.
**摘要**：使用RNF5抗体进行蛋白质印迹分析，发现RNF5通过泛素化线粒体融合蛋白MFN2.调控线粒体分裂和神经元凋亡，为神经退行性疾病机制研究提供新方向。
4. **文献名称**：*Antiviral role of RNF5 in STING-mediated innate immune signaling*
**作者**：Wang, C., et al.
**摘要**：通过RNF5抗体检测蛋白表达，研究揭示RNF5通过泛素化修饰STING蛋白，负调控抗病毒天然免疫信号通路，影响宿主对RNA病毒感染的应答。
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**注**：以上文献为示例，实际引用需根据具体研究领域检索(如PubMed或Google Scholar)。建议补充发表年份及期刊名称以完善信息。
The RNF5 antibody is a crucial tool for studying the Ring Finger Protein 5 (RNF5), an E3 ubiquitin ligase involved in the ubiquitin-proteasome system. RNF5. also known as RMA1. plays a key role in endoplasmic reticulum-associated degradation (ERAD), targeting misfolded or damaged proteins for proteasomal degradation. It contains a conserved RING domain critical for its ligase activity and is localized to the endoplasmic reticulum (ER) and mitochondria-associated membranes (MAMs), where it regulates stress responses, protein quality control, and cellular homeostasis. RNF5 substrates include proteins like HSP70. CFTR, and PDIA3. linking it to diseases such as cystic fibrosis, cancer, and neurodegenerative disorders.
Antibodies against RNF5 are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to assess its expression, subcellular localization, and interactions. Research highlights RNF5's dual roles in cancer—acting as both a tumor suppressor (by degrading oncoproteins) and promoter (via modulating metastasis-related pathways). It also influences mitochondrial dynamics and autophagy, connecting it to Parkinson’s and Alzheimer’s diseases.
The development of specific RNF5 antibodies has advanced studies on ER stress, unfolded protein response, and therapeutic targeting of protein degradation pathways. Validated antibodies are essential for elucidating RNF5's regulatory mechanisms and its potential as a biomarker or therapeutic target in diverse pathologies.
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