| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | NOS; INOS; NOS2A; HEP-NOS |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human NOS2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NOS2(iNOS)抗体的3篇参考文献示例，格式为文献名称、作者及摘要概述：
1. **"Selective inhibition of inducible nitric oxide synthase by antisense oligonucleotides in human astrocytes"**
*作者：Xie Q. et al.*
摘要：研究利用NOS2抗体验证siRNA敲低效果，证实iNOS在炎症性神经退行性疾病中的调控作用，抗体用于Western blot检测人星形胶质细胞模型中的蛋白表达水平。
2. **"Role of inducible nitric oxide synthase in colorectal cancer progression"**
*作者：Ambs S. et al.*
摘要：通过免疫组化分析结直肠癌组织中iNOS表达水平，使用特异性NOS2抗体证实其高表达与肿瘤血管生成及患者预后不良相关。
3. **"Comparative evaluation of commercial antibodies for the detection of iNOS in murine macrophages"**
*作者：Alderton W.K. et al.*
摘要：系统比较多种市售NOS2抗体的特异性，通过LPS诱导的巨噬细胞模型验证抗体在流式细胞术和免疫荧光中的适用性，推荐高信噪比抗体型号。
注：以上文献信息为示例性质，具体引用时请核对真实文献的标题、作者及内容。建议通过PubMed或Web of Science以关键词“iNOS antibody application”或“NOS2 immunohistochemistry”检索最新研究。
Nitric oxide synthase 2 (NOS2), also known as inducible NOS (iNOS), is an enzyme encoded by the NOS2 gene that catalyzes the production of nitric oxide (NO) from L-arginine. Unlike constitutive isoforms (NOS1 and NOS3), NOS2 is not typically expressed under normal physiological conditions but is induced in response to inflammatory stimuli, such as cytokines (e.g., TNF-α, IFN-γ) or microbial pathogens. This induction occurs primarily in immune cells (e.g., macrophages), fibroblasts, and epithelial cells, where NO acts as a key mediator of antimicrobial defense, vasodilation, and immune regulation. However, dysregulated or prolonged NOS2 expression has been implicated in chronic inflammation, autoimmune diseases, and cancer progression.
NOS2 antibodies are essential tools for detecting and quantifying NOS2 protein expression in research. They are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to study NOS2's role in diseases such as sepsis, rheumatoid arthritis, neurodegenerative disorders, and atherosclerosis. These antibodies target specific epitopes of the NOS2 protein, often distinguishing it from other NOS isoforms. Validation of antibody specificity is critical, as cross-reactivity or batch variability can affect experimental outcomes. Commercial NOS2 antibodies are typically raised against human, mouse, or rat NOS2 sequences, enabling species-specific applications. Recent studies also explore NOS2 inhibition as a therapeutic strategy, further driving demand for reliable antibodies in mechanistic and drug development research.
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