| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CHC1; RCC1-I; SNHG3-RCC1 |
| WB Predicted band size | 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human RCC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于RCC1抗体的参考文献示例(注：部分文献信息为模拟概括，实际引用请核实原文)：
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1. **文献名称**: "Regulator of Chromosome Condensation 1 (RCC1) is essential for mitotic progression in human cells"
**作者**: Hutchins, J.R. et al.
**摘要**: 本研究利用RCC1特异性抗体进行免疫荧光染色和蛋白质印迹分析，发现RCC1在有丝分裂中通过调节染色体结构和纺锤体组装维持基因组稳定性。敲低RCC1导致染色体分离异常和细胞周期停滞。
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2. **文献名称**: "Interaction between RCC1 and Ran-GTPase in nuclear transport regulation"
**作者**: Ohtsubo, M. et al.
**摘要**: 通过免疫共沉淀(Co-IP)结合RCC1抗体，揭示了RCC1与Ran-GTP酶的相互作用机制，证明RCC1通过调控Ran的GTP/GDP循环参与核质运输和细胞周期调控。
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3. **文献名称**: "RCC1 expression correlates with tumor progression in renal cell carcinoma"
**作者**: Moore, W. et al.
**摘要**: 采用RCC1抗体对肾细胞癌组织进行免疫组化分析，发现RCC1蛋白表达水平与肿瘤分期和转移显著相关，提示其作为潜在生物标志物的价值。
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4. **文献名称**: "Characterization of a monoclonal antibody against human RCC1 for functional studies"
**作者**: Tanaka, K. & Nishimoto, T.
**摘要**: 报道了一种高特异性抗RCC1单克隆抗体的开发，验证其在流式细胞术、Western blot和活细胞成像中的应用，证明其可用于研究RCC1的亚细胞定位和动态功能。
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(以上内容基于领域内典型研究方向模拟，具体文献需通过学术数据库检索确认。)
The Regulator of Chromosome Condensation 1 (RCC1) is a critical protein involved in cell cycle regulation, primarily functioning as a guanine nucleotide exchange factor (GEF) for the Ras-related nuclear (Ran) GTPase. RCC1 binds to chromatin through its interaction with histones and is essential for maintaining the Ran-GTP/Ran-GDP gradient, which governs nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope formation during mitosis. Its seven-bladed β-propeller structure enables dynamic association with chromatin, facilitating spatial regulation of Ran-GTP production.
RCC1 antibodies are widely used tools to study its expression, localization, and function in cellular processes. These antibodies are crucial for applications such as Western blotting, immunofluorescence, and immunoprecipitation to investigate RCC1's role in cell cycle progression, genome stability, and cancer biology. Dysregulation of RCC1 has been linked to chromosomal missegregation, aneuploidy, and tumorigenesis, making its detection relevant in cancer research. Validated RCC1 antibodies typically exhibit high specificity, recognizing conserved epitopes across species, and are employed in both experimental and diagnostic contexts to explore correlations between RCC1 dysfunction and diseases like renal cell carcinoma or leukemia. Their utility extends to screening therapeutic agents targeting cell cycle anomalies.
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