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产品描述：小鼠Dickkopf相关蛋白3(DKK3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DKK3含量。DKK3作为靶点备受关注。其背景上，它在多种生理和病理过程中发挥作用。研究机制方面，主要聚焦于其在信号通路中的调控作用，如影响细胞增殖、分化等过程。探索DKK3相关机制，对理解疾病发生发展及开发针对性治疗策略有重要意义。试剂盒检测范围为9.4 pg/mL-600 pg/mL，适用于微量样本的精准检测；适用于体外培养细胞上清、动物模型组织提取物等多种科研场景；为探索DKK3在疾病机制、药物靶点筛选及分子调控网络研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Dkk3Dickkopf-related protein 3 ELISA Kit; Dickkopf-3 ELISA Kit; Dkk-3 ELISA Kit; mDkk-3 ELISA Kit
缩写：DKK3
Uniprot No.：Q9QUN9
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：9.38 pg/ml-600 pg/ml
灵敏度：2.34 pg/ml
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse DKK3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-104 | |||||
| 1:4 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 100-109 | |||||
回收率：
| The recovery of mouse DKK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-96 | ||||
| EDTA plasma (n=4) | 93 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 6002.2892.4872.3882.204 3001.5821.5321.5571.373 1500.8810.8980.8900.706 750.5300.5020.5160.332 37.50.3820.3750.3790.195 18.80.3120.3060.3090.125 9.40.2510.2480.2500.066 00.1820.1860.184 | ||||||
货期：3-5 working days
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