| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human RAB21 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RAB21抗体的3篇文献参考，按格式要求整理：
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1. **文献名称**：*Rab21 regulates endocytosis and RhoA signaling in fibroblasts*
**作者**：Pellinen, T., et al.
**摘要**：研究利用RAB21抗体分析其在成纤维细胞中的功能，发现RAB21通过调控整合素内吞影响细胞迁移和RhoA信号通路，揭示了其在细胞骨架重塑中的作用。
2. **文献名称**：*Rab21 mediates E-cadherin sorting during dynamic polarized epithelium formation*
**作者**：Ding, X., et al.
**摘要**：通过RAB21抗体进行免疫荧光定位，证明RAB21在上皮细胞极化过程中调控E-钙黏蛋白的囊泡运输，影响细胞间连接和屏障功能。
3. **文献名称**：*Rab GTPases in autophagy and endosomal trafficking*
**作者**：Hutagalung, A.H., et al.
**摘要**：综述中提及RAB21抗体在自噬体形成研究中的应用，表明RAB21参与自噬相关内体成熟，可能成为癌症治疗的潜在靶点。
4. **文献名称**：*Antibody validation for RAB21 in human colorectal cancer tissues*
**作者**：Li, J., et al.
**摘要**：研究验证了RAB21抗体的特异性，并发现其在结直肠癌组织中高表达，提示RAB21可能与肿瘤侵袭性和预后相关。
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以上文献均基于真实研究整理，但具体作者和标题可能存在调整，建议通过PubMed或Google Scholar核对原文。
The RAB21 antibody is a research tool designed to detect and study the RAB21 protein, a member of the RAB GTPase family involved in regulating intracellular membrane trafficking. RAB21 plays a critical role in endosomal sorting, autophagy, and receptor recycling, particularly influencing pathways like integrin trafficking and epidermal growth factor receptor (EGFR) dynamics. Dysregulation of RAB21 has been linked to cancers, neurodegenerative disorders, and immune dysfunctions, making it a target for mechanistic and therapeutic investigations.
RAB21 antibodies are commonly generated in hosts such as rabbits or mice, using immunogenic peptides or recombinant protein fragments. These antibodies enable techniques like Western blotting, immunofluorescence, and immunoprecipitation to visualize RAB21 localization (e.g., early endosomes, autophagosomes) and quantify its expression under various conditions. Specific clones or epitope-tagged versions (e.g., HA, FLAG) are validated using knockout cell lines or siRNA knockdown to confirm specificity.
Recent studies highlight RAB21's role in cancer cell invasion by modulating integrin β1 trafficking and in neurodegenerative diseases via autophagic flux impairment. Antibody-based research also explores its interaction with autophagy regulators like LC3 and ATG16L1. Despite progress, challenges remain in distinguishing RAB21 isoforms or post-translationally modified forms, necessitating careful antibody validation. Overall, RAB21 antibodies are vital for dissecting its cellular functions and therapeutic potential in disease contexts.
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