| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MEL; RAB8 |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human RAB8A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RAB8A抗体的3篇示例参考文献(注：以下为虚构示例，仅供参考格式)：
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1. **文献名称**: "RAB8A regulates vesicular transport in polarized epithelial cells"
**作者**: Smith J, et al.
**摘要**: 本研究利用特异性RAB8A抗体，通过免疫荧光和Western blot分析，揭示了RAB8A在极化上皮细胞中调控囊泡从高尔基体到基底侧膜运输的关键作用，并证实其与细胞极性蛋白的相互作用。
2. **文献名称**: "RAB8A dysfunction links to ciliopathies in a zebrafish model"
**作者**: Chen L, et al.
**摘要**: 通过RAB8A抗体标记，作者发现RAB8A缺失导致斑马鱼纤毛结构异常，证明其在纤毛形成中的必要性，并提示其突变可能与人类纤毛病相关。
3. **文献名称**: "RAB8A interacts with optineurin in autophagy pathways"
**作者**: Tanaka K, et al.
**摘要**: 该研究使用RAB8A抗体进行免疫共沉淀实验，发现RAB8A与自噬相关蛋白optineurin的结合，揭示其在细胞自噬和蛋白质降解通路中的新功能。
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**说明**：以上文献为示例，实际引用时需通过PubMed、Web of Science等平台检索真实文献，并核对抗体货号(如Santa Cruz的sc-12922或Abcam的ab188574)及实验应用场景。
The RAB8A antibody is a crucial tool for studying the RAB8A protein, a member of the Ras-related small GTPase family involved in membrane trafficking and cellular dynamics. RAB8A regulates vesicular transport from the Golgi apparatus to the plasma membrane, playing a key role in exocytosis, ciliogenesis, and maintaining cell polarity. Dysregulation of RAB8A is linked to ciliopathies, neurodegenerative disorders (e.g., Parkinson’s disease), and cancer metastasis, making it a focus of pathophysiological research.
RAB8A antibodies, typically raised in rabbits or mice as monoclonal or polyclonal forms, enable the detection and localization of endogenous or exogenous RAB8A in techniques like Western blotting, immunofluorescence, and immunohistochemistry. These antibodies often target specific epitopes within conserved regions of RAB8A, such as its GTP-binding domains or C-terminal regions, allowing researchers to investigate its activation state (GTP- vs. GDP-bound) and interactions with effector proteins like optineurin or the exocyst complex.
Validated RAB8A antibodies are essential for studying its role in cellular processes, such as secretory vesicle trafficking, autophagy, and primary cilium formation. Commercial antibodies are rigorously tested for specificity (e.g., via knockout cell lines) and reproducibility, ensuring reliable data. Researchers also utilize these antibodies to explore disease mechanisms, such as impaired ciliary function in Joubert syndrome or RAB8A’s involvement in α-synuclein aggregation in Parkinson’s disease, underscoring their versatility in both basic and translational studies.
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