首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人双氢睾酮(DHT)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DHT含量。DHT即双氢睾酮，是睾酮经5α-还原酶作用转化而来。它与雄激素性脱发、前列腺增生等密切相关。研究机制主要聚焦于其与雄激素受体结合，影响毛囊、前列腺等组织细胞的生长、分化，为相关疾病的药物研发提供方向。试剂盒检测范围为10 pg/mL-2000 pg/mL，本产品可广泛应用于激素作用机制研究、内分泌系统功能评估、前列腺相关疾病模型构建及药物干预效果分析等科研领域，为探索DHT在生理病理过程中的调控网络提供高效工具，适用于细胞实验、动物模型及临床前研究的数据获取。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：DHT
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：10 pg/mL-2000 pg/mL
灵敏度：5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 86-105 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-103 | |||||
回收率：
| The recovery of human DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-99 | ||||
| EDTA plasma (n=4) | 97 | 92-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 20000.1790.1850.182 5000.3130.3190.316 1600.5230.5290.526 400.8180.8020.810 101.3171.3301.324 01.9521.9431.948 | ||||||
货期：3-5 working days
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