首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人沙漠刺猬因子(DHH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的DHH含量。靶点研究是药物研发的关键，旨在识别与疾病相关的生物分子，如蛋白质、酶或信号通路。通过理解靶点的作用机制，研究人员可设计针对这些靶点的药物，以治疗疾病。靶点研究涉及基因突变分析、信号通路解析和药物筛选等多个环节。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，支持科研领域对DHH蛋白表达水平的精准分析，例如基础研究中信号通路机制探索、疾病模型中DHH动态变化的系统性研究，或在药物开发过程中评估干预措施对Hedgehog通路的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C78960 ELISA Kit; Desert hedgehog (Drosophila) homolog ELISA Kit; Desert hedgehog ELISA Kit; Desert hedgehog homolog (Drosophila) ELISA Kit; Desert hedgehog protein C-product ELISA Kit; Desert hedgehog protein precursor ELISA Kit; DHH ELISA Kit; DHH_HUMAN ELISA Kit; GDXYM ELISA Kit; HHG-3 ELISA Kit; Hira ELISA Kit; SRXY7 ELISA Kit
缩写：DHH
Uniprot No.：O43323
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DHH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 89 | ||||
| Range % | 84-92 | |||||
| 1:10 | Average % | 99 | ||||
| Range % | 95-101 | |||||
| 1:20 | Average % | 99 | ||||
| Range % | 95-102 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of human DHH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-96 | ||||
| EDTA plasma (n=4) | 96 | 94-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4182.4022.4102.2975001.7661.8801.8231.7102501.2231.2131.2181.1051250.6890.7070.6980.58562.50.4530.4320.4430.33031.20.3240.3310.3280.21515.60.2240.2180.2210.10800.1150.1110.113 | ||||||
货期：3-5 working days
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