| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase |
| WB Predicted band size | 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human PPM1F |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于PPM1F抗体的3篇参考文献示例(内容为模拟生成，仅供参考)：
---
1. **文献名称**：*PPM1F regulates AMPK signaling and mitochondrial dynamics in neurons*
**作者**：Tanaka S. et al.
**摘要**：本研究探讨了PPM1F蛋白磷酸酶在神经元代谢中的作用，通过Western blot和免疫组化技术，利用PPM1F抗体验证了其在AMPK通路中的调控功能。结果显示，PPM1F缺失导致线粒体分裂异常，提示其在神经退行性疾病中的潜在机制。
2. **文献名称**：*Downregulation of PPM1F in breast cancer promotes cell invasion via MAPK pathway*
**作者**：Zhang J. et al.
**摘要**：该研究分析了PPM1F在乳腺癌中的表达及其临床意义。通过免疫荧光和免疫沉淀(使用PPM1F特异性抗体)，发现PPM1F低表达与MAPK信号激活相关，并促进癌细胞侵袭，提示其作为肿瘤抑制因子的作用。
3. **文献名称**：*Generation and validation of a PPM1F knockout mouse model using CRISPR/Cas9*
**作者**：Chen L. et al.
**摘要**：研究团队利用CRISPR/Cas9技术构建了PPM1F敲除小鼠模型，并通过Western blot(使用PPM1F抗体)及qPCR验证了基因敲除效率。模型显示心脏发育异常，表明PPM1F在心肌细胞分化中的关键调控作用。
---
以上文献摘要均围绕PPM1F抗体的应用(如蛋白表达验证、功能机制研究)展开，涵盖神经科学、癌症生物学及模型构建领域。如需真实文献，建议通过PubMed或Google Scholar检索关键词“PPM1F antibody”或“PPM1F function”。
The PPM1F antibody is a research tool used to detect and study the protein phosphatase 1F (PPM1F), a member of the metal-dependent protein phosphatase (PPM) family. Also known as POPX2. PPM1F is a serine/threonine phosphatase involved in regulating cellular stress responses, energy metabolism, and apoptosis. It interacts with key signaling pathways, including the AMP-activated protein kinase (AMPK) and Ca²⁺/calmodulin-dependent protein kinase kinase β (CaMKKβ) pathways, modulating processes such as autophagy, mitochondrial function, and cell survival. Structurally, PPM1F contains a conserved catalytic domain critical for its phosphatase activity and a regulatory C-terminal domain that influences substrate specificity.
Researchers employ PPM1F antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to quantify protein expression, assess localization, and investigate its role in disease contexts. Studies have linked PPM1F dysregulation to cancer, metabolic disorders, and neurodegenerative diseases, though its exact role varies by cellular context. For example, PPM1F may act as a tumor suppressor by dephosphorylating AMPK to promote energy homeostasis or as a potential oncogene in certain malignancies. The antibody’s utility in distinguishing these dual roles underscores its importance in elucidating PPM1F’s complex regulatory mechanisms and therapeutic potential. Commercial antibodies are typically validated for specificity using knockout cell lines or siRNA knockdown to ensure reliable experimental outcomes.
×
