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产品描述：犬双氢睾酮(DHT)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DHT含量。DHT即双氢睾酮，是睾酮在5α-还原酶作用下转化而来。它与雄激素性脱发、前列腺增生等疾病密切相关。研究机制方面，主要聚焦其和靶细胞内雄激素受体结合，影响基因表达，促进细胞生长分化，目前也是相关疾病药物研发重要靶点。试剂盒检测范围为40 pg/mL-8000 pg/mL，该产品适用于动物实验中对激素干预效果评估、生殖健康研究或代谢性疾病模型的 DHT 动态分析，例如前列腺增生、脱发机制或内分泌紊乱相关研究，为探索犬类生理病理机制提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：DHT
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：40 pg/mL-8000 pg/mL
灵敏度：20 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 93-103 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-104 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 82-95 | |||||
回收率：
| The recovery of canine DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 99 | 98-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average80000.2240.2230.22420000.3620.3560.3596400.5640.5570.5611600.8190.8040.812401.1741.2751.22502.3052.4042.355 | ||||||
货期：3-5 working days
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