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产品描述：人Dickkopf相关蛋白2(DKK2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的DKK2含量。DKK2，全称dickkopf WNT signaling pathway inhibitor 2，是一种分泌蛋白，能够抑制β-联蛋白介导的Wnt信号传导，并具有促血管生成活性。其在多种肿瘤组织中表达上调，通过抑制杀伤性免疫细胞激活和减弱肿瘤血管生成来抑制肿瘤形成。研究显示，DKK2的抑制作用与CD8+细胞毒性T淋巴细胞(CTL)活性和肿瘤血管生成减弱有关，是治疗癌症的有希望靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外培养细胞模型、动物组织或体液样本的DKK2蛋白定量研究；为探索Wnt信号通路在癌症、骨疾病、干细胞分化等领域的分子机制提供可靠工具，满足基础科研中对蛋白表达动态分析的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Dickkopf 2 ELISA Kit; Dickkopf gene 2 ELISA Kit; Dickkopf homolog 2 ELISA Kit; Dickkopf related protein 2 ELISA Kit; Dickkopf WNT signaling pathway inhibitor 2 ELISA Kit; Dickkopf-2 ELISA Kit; Dickkopf-related protein 2 ELISA Kit; Dickkopf2 ELISA Kit; DKK 2 ELISA Kit; Dkk-2 ELISA Kit; Dkk2 ELISA Kit; DKK2_HUMAN ELISA Kit; hDkk 2 ELISA Kit; hDkk-2 ELISA Kit; hDkk2 ELISA Kit
缩写：DKK2
Uniprot No.：Q9UBU2
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DKK2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 80-94 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human DKK2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 98 | 95-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.4162.4562.4362.324101.5791.6851.6321.52050.8340.9010.8680.7562.50.5150.5450.5300.4181.250.3720.3650.3690.2570.6250.2660.2550.2610.1490.3120.1840.1780.1810.06900.1130.1110.112 | ||||||
货期：3-5 working days
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