| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RDH54 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human RAD54B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RAD54B抗体的3篇代表性文献示例(注：以下信息为模拟示例，实际文献需通过学术数据库验证)：
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1. **文献名称**: *RAD54B promotes homologous recombination repair and regulates genomic stability in human cells*
**作者**: Taniguchi T, et al.
**摘要**: 研究利用RAD54B特异性抗体，通过免疫沉淀和免疫荧光技术，揭示RAD54B在同源重组修复(HR)中的作用。实验表明，RAD54B缺失会导致DNA双链断裂修复缺陷，并增加染色体畸变风险。
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2. **文献名称**: *Expression of RAD54B in colorectal cancer and its clinical significance*
**作者**: Wang L, et al.
**摘要**: 通过免疫组化(使用抗RAD54B抗体)分析结直肠癌组织中RAD54B的表达水平，发现其高表达与患者生存率降低和化疗耐药性相关，提示RAD54B可能作为癌症预后标志物。
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3. **文献名称**: *Functional characterization of RAD54B mutations in Fanconi anemia patients*
**作者**: Kim Y, et al.
**摘要**: 利用Western blot(抗RAD54B抗体)和基因编辑技术，发现范可尼贫血患者的RAD54B突变会导致同源重组功能异常，为疾病机制提供分子层面的解释。
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**提示**：
- 实际文献可通过PubMed、Google Scholar等平台搜索关键词“RAD54B antibody”或“RAD54B function”获取。
- 部分研究可能侧重于抗体开发(如用于诊断)，或通过抗体探究RAD54B在癌症、基因组稳定性中的功能。
- 建议结合具体研究领域筛选文献(如肿瘤学、DNA修复机制等)。
RAD54B antibody is a specialized tool used to detect and study the RAD54B protein, a key player in DNA repair and homologous recombination. Belonging to the RAD54 family of DNA-dependent ATPases, RAD54B facilitates genomic stability by working alongside RAD51 to repair double-strand breaks (DSBs) through the homologous recombination repair (HRR) pathway. This protein enhances RAD51’s ability to form nucleoprotein filaments on single-stranded DNA, promoting strand pairing and exchange—a critical step in error-free DNA repair. Dysregulation of RAD54B is linked to genomic instability, carcinogenesis, and chemotherapy resistance, making it a focus in cancer research, particularly in BRCA-mutant cancers with defective HRR.
The RAD54B antibody is widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and interactions in various tissues and cancer models. Studies leveraging this antibody have revealed RAD54B’s roles in meiosis, somatic cell proliferation, and tumor suppression. Its expression is often downregulated in cancers such as colorectal and breast carcinomas, correlating with poor prognosis. Additionally, RAD54B antibodies aid in exploring synthetic lethality strategies, where inhibiting alternative repair pathways in HRR-deficient cells could enhance therapeutic outcomes. Researchers also investigate its potential as a biomarker for predicting treatment responses in DNA-damaging therapies. Overall, RAD54B antibodies are vital for advancing our understanding of DNA repair mechanisms and developing targeted cancer therapies.
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