首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠delta蛋白同源物1(DLK1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DLK1含量。DLK1是一个重要靶点。它在胚胎发育中发挥作用，且与多种疾病发生发展相关。研究机制方面，主要聚焦于其信号通路以及与其他分子的相互作用，了解其如何调控细胞增殖、分化等过程，为疾病治疗靶点开发提供依据。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于小鼠模型中DLK1蛋白表达的动态监测，可广泛用于脂肪代谢研究、发育生物学机制探索、肿瘤微环境分析等科研领域，为探究DLK1在生理及病理过程中的调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Dlk1 ELISA kit; Dlk ELISA kit; Pref1 ELISA kit; Scp-1Protein delta homolog 1 ELISA kit; DLK-1 ELISA kit; Adipocyte differentiation inhibitor protein ELISA kit; Preadipocyte factor 1 ELISA kit; Pref-1) [Cleaved into: Fetal antigen 1 ELISA kit; FA1)] ELISA kit
缩写：DLK1
Uniprot No.：Q09163
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse DLK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-96 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 92-108 | |||||
回收率：
| The recovery of mouse DLK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-102 | ||||
| EDTA plasma (n=4) | 89 | 82-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.0451.9441.9951.8445001.6441.5231.5841.4332501.0731.0521.0630.9121250.6730.6620.6680.51762.50.3710.3820.3770.22631.20.2840.2730.2790.12815.60.2350.2310.2330.08200.1480.1530.151 | ||||||
货期：3-5 working days
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