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产品描述：人δ样蛋白3(DLL3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DLL3含量。DLL3（δ样蛋白3）是一种在多种肿瘤组织中异常高表达的膜蛋白，正常组织中表达有限，这使其成为有潜力的肿瘤治疗靶点。研究主要围绕阻断其与 Notch 信号通路相互作用，抑制肿瘤细胞增殖、存活、迁移等，为癌症治疗提供新思路。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于基础科研中探索DLL3在肿瘤微环境、干细胞分化或疾病生物标志物筛选等方向的作用机制，为体外研究提供可靠的蛋白表达水平数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Delta Drosophila like 3 ELISA Kit; Delta like 3 Drosophila ELISA Kit; Delta like 3 homolog Drosophila ELISA Kit; Delta like 3 protein ELISA Kit; Delta like protein 3 precursor ELISA Kit; Delta-like protein 3 ELISA Kit; Delta3 ELISA Kit; Dll3 ELISA Kit; DLL3_HUMAN ELISA Kit; Drosophila Delta homolog 3 ELISA Kit; SCDO1 ELISA Kit; SCOD1 ELISA Kit; Spondylocostal dysostosis autosomal recessive ELISA Kit
缩写：DLL3
Uniprot No.：Q9NYJ7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Developmental Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DLL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 105 | ||||
| Range % | 100-112 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:20 | Average % | 93 | ||||
| Range % | 89-100 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human DLL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-93 | ||||
| EDTA plasma (n=4) | 96 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12002.1992.3542.2772.1346001.7141.8111.7631.6203001.1721.1041.1380.9951500.6980.6740.6860.543750.4250.4070.4160.27337.50.3100.3290.3200.17718.750.2070.2190.2130.07000.1410.1450.143 | ||||||
货期：3-5 working days
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