首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人δ样蛋白4(DLL4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DLL4含量。DLL4是重要的靶点，在Notch信号通路中发挥关键作用，对血管生成等生理过程有调节功能。研究发现其异常激活与肿瘤血管异常及生长相关。当前研究主要聚焦通过抑制DLL4调控肿瘤血管生成，为抗肿瘤治疗提供新思路。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，为研究其在血管形成机制、肿瘤微环境调控或相关信号通路互作提供可靠工具；科研人员可利用该产品开展体外模型、动物实验或疾病样本中DLL4的动态表达研究，尤其适用于探索血管异常相关疾病的分子机制或药物干预效果的评估；是基础研究与转化医学领域的高效解决方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AOS6 ELISA Kit; Delta 4 ELISA Kit; delta 4 precursor ELISA Kit; Delta ligand 4 ELISA Kit; delta ligand 4 precursor ELISA Kit; Delta like 4 ELISA Kit; Delta like 4 homolog ELISA Kit; Delta like 4 protein ELISA Kit; Delta like canonical Notch ligand 4 ELISA Kit; Delta like protein 4 ELISA Kit; Delta-like 4 (Drosophila) ELISA Kit; Delta-like protein 4 ELISA Kit; Delta4 ELISA Kit; DLL 4 ELISA Kit; Dll4 ELISA Kit; DLL4_HUMAN ELISA Kit; Drosophila Delta homolog 4 ELISA Kit; hdelta2 ELISA Kit; Homeobox protein DLL-4 ELISA Kit; MGC126344 ELISA Kit; Notch ligand delta 2 ELISA Kit; Notch ligand DLL4 ELISA Kit; Notch ligand DLL4 precursor ELISA Kit; XDLL-4 ELISA Kit
缩写：DLL4
Uniprot No.：Q9NR61
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Developmental Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DLL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 81-89 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 83-90 | |||||
回收率：
| The recovery of human DLL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-99 | ||||
| EDTA plasma (n=4) | 100 | 96-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.2612.2982.2802.0987501.5751.5951.5851.4033750.9890.9750.9820.800187.50.6430.6060.6250.443940.4340.4290.4320.250470.3170.3380.3280.14623.50.2670.2780.2730.09100.1810.1830.182 | ||||||
货期：3-5 working days
×
