首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人皮肤蛋白(DPT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的DPT含量。人皮肤蛋白DPT作为一种重要的细胞外基质蛋白，其研究机制主要涉及其在皮肤纤维化、细胞粘附和信号传导中的作用。DPT通过整合素与细胞表面结合，促进细胞粘附，增强TGFβ1活性，抑制细胞增殖，促进胶原纤维形成，并在低温下稳定胶原纤维。此外，DPT还与皮肤纤维化、创面愈合和衰老皮肤相关。试剂盒检测范围为0.312 ng/mL-20 ng/mL，支持科研人员探究皮肤疾病模型（如瘢痕形成、硬皮病）中DPT的动态变化，评估药物干预对皮肤纤维化通路的影响，或分析体外培养的成纤维细胞等模型中DPT的分泌水平。适用于基础医学、药理学及皮肤生物学领域探索DPT在组织再生、细胞外基质重塑等方向的功能机制，为相关疾病靶点发现及治疗策略开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DERM_HUMAN ELISA Kit; Dermatopontin ELISA Kit; Dpt ELISA Kit; TRAMP ELISA Kit; Tyrosine rich acidic matrix protein ELISA Kit; Tyrosine-rich acidic matrix protein ELISA Kit
缩写：DPT
Uniprot No.：Q07507
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DPT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-102 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 82-103 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 86-108 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-105 | |||||
回收率：
| The recovery of human DPT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 83-104 | ||||
| EDTA plasma (n=4) | 95 | 87-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.4172.4932.4552.314101.7631.8171.7901.64951.0250.9821.0040.8632.50.6110.5950.6030.4621.250.4360.4160.4260.2850.6250.3290.3070.3180.1770.3120.2380.2280.2330.09200.1420.1390.141 | ||||||
货期：3-5 working days
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