| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | TAP; Sca1; Sca-1; Ly-6A.2; Ly-6A/E; Ly-6E.1 |
| WB Predicted band size | 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | Synthetic peptide of mouse Ly6a |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于Ly6a(Sca-1)抗体的3篇代表性文献及其摘要内容的简要整理：
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1. **文献名称**: *Sca-1 as a marker of mesenchymal stem cells in mice*
**作者**: Holmes C, Stanford WL
**摘要**: 该综述讨论了Sca-1(Ly6a)作为小鼠间充质干细胞(MSCs)表面标记物的应用，分析了其在干细胞分选中的特异性及局限性，并探讨了Sca-1在干细胞自我更新和分化中的潜在功能。
2. **文献名称**: *Antibody binding to murine Ly6a (Sca-1) characterizes a subset of hematopoietic stem cells with distinct regenerative potential*
**作者**: Ma X et al.
**摘要**: 通过比较不同Sca-1抗体克隆(如D7、E13-161.7)对小鼠造血干细胞(HSCs)的功能影响，研究发现某些克隆可能导致细胞活化和归巢能力差异，提示抗体选择可能影响实验结果的准确性。
3. **文献名称**: *Sca-1⁺ cells in the mammary gland represent a progenitor cell population*
**作者**: Welm BE et al.
**摘要**: 研究揭示了乳腺组织中Sca-1⁺细胞的干细胞特性，发现Ly6a抗体可用于分离具有自我更新和分化潜能的乳腺上皮祖细胞，为肿瘤干细胞研究提供了工具。
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**备注**：以上文献为示例，实际引用时需核对作者、标题及期刊信息准确性。建议通过PubMed或Google Scholar以关键词“Ly6a antibody”“Sca-1 function”等检索最新研究。
Ly6 antibodies target members of the Ly6 (Lymphocyte Antigen 6) protein family, a group of evolutionarily conserved cell-surface glycoproteins characterized by a conserved LU domain structure. These proteins are typically anchored to the plasma membrane via glycosylphosphatidylinositol (GPI) and play diverse roles in immune regulation, cell signaling, and stem cell maintenance. In mammals, the Ly6 family includes numerous members, such as Sca-1 (stem cell antigen-1) in mice and LY6E, LY6K, or LY6D in humans.
Ly6 antibodies are widely used in research to identify and isolate specific cell populations. For example, anti-Sca-1 antibodies are critical for identifying murine hematopoietic stem cells and progenitor cells. In humans, LY6 antibodies help study immune cell subsets, cancer biology (e.g., LY6K overexpression in tumors), and viral entry mechanisms (e.g., LY6E's role in modulating viral infection).
The Ly6 family's involvement in diseases, particularly cancer and immune disorders, has driven interest in therapeutic applications. Antibodies against Ly6 proteins are being explored for targeted therapies, diagnostics, and as biomarkers. However, functional redundancy and species-specific differences (e.g., Sca-1 has no direct human ortholog) pose challenges in translational research. Overall, Ly6 antibodies remain vital tools for dissecting cellular heterogeneity and disease mechanisms.
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