首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠雌激素(E)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的E含量。试剂盒检测范围为25 pg/mL-1000 pg/mL，适用于科研场景如卵巢功能研究、激素替代治疗模型验证、环境污染物对内分泌干扰效应评估等，还可用于生殖生物学研究、内分泌疾病模型构建及环境激素效应评估等。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：E
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：25 pg/mL-1000 pg/mL
灵敏度：25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse estrogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 86-98 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 95-105 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 88-92 | |||||
回收率：
| The recovery of mouse estrogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 96 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 02.4112.3972.404 251.4851.5261.506 750.9000.8580.879 2300.5640.5770.571 5000.3860.3640.375 10000.1820.1830.183 | ||||||
本试剂盒所含材料：
A 96-well Assay plate--The 96-well plate has been pre-coated with goat-anti-rabbit estrogen antibody.
Standard (6 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
HRP-conjugated estrogen(1 x 6 ml) --Bind to the anti-mouse estrogen antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
Anti-mouse estrogen Antibody (1 x 6 ml)--React with both estrogen in the samples/standards and HRP-conjugated estrogen.
Wash Buffer (20x concentrate) 1 x 15ml --Wash away unbound or free substances.
Substrate A (1 x 7 ml) --Mix with Substrate B and interacts with HRP, eliciting the solution turns blue.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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