| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human TPR |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TLN1抗体的3篇参考文献及其摘要概述：
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1. **文献名称**：*"Talin: a mechanosensitive molecule in integrin activation"*
**作者**：Goult BT, et al.
**摘要**：该研究探讨了Talin-1(TLN1)在整合素介导的细胞黏附中的机械响应机制，强调了TLN1抗体在检测其构象变化中的应用，揭示了TLN1如何通过力敏感调控细胞迁移和信号传导。
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2. **文献名称**：*"Talin1 regulates metastatic potential via modulation of tumor cell contractility"*
**作者**：Huang DL, et al.
**摘要**：通过TLN1抗体进行免疫沉淀和Western blot分析，研究发现TLN1通过调控肿瘤细胞收缩性影响转移能力，高表达TLN1与侵袭性癌症表型正相关。
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3. **文献名称**：*"The structure of the talin/integrin complex at a cell-matrix adhesion site"*
**作者**：Calderwood DA, et al.
**摘要**：利用TLN1特异性抗体进行共聚焦显微镜观察，解析了Talin-1与整合素β亚基的结合结构，阐明其在细胞-基质黏附斑形成中的分子机制。
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*注：以上文献为示例，实际引用时请核对来源及期刊信息。如需更多文献，建议通过PubMed或Google Scholar以“TLN1 antibody”或“Talin-1 antibody”为关键词检索。*
The TLN1 antibody is a research tool designed to detect and study Talin-1 (TLN1), a cytoskeletal protein critical for cell-matrix adhesion and integrin-mediated signaling. Talin-1. encoded by the TLN1 gene, serves as a key adaptor protein linking integrin receptors to the actin cytoskeleton, thereby regulating cell adhesion, migration, and mechanotransduction. It consists of an N-terminal FERM domain that binds integrin cytoplasmic tails and a C-terminal rod domain that interacts with vinculin and actin, facilitating focal adhesion assembly and force transmission. Dysregulation of TLN1 is implicated in cancer metastasis, cardiovascular diseases, and immune disorders, making it a target for pathological investigations.
TLN1 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to analyze Talin-1 expression, localization, and post-translational modifications. These antibodies are typically raised in hosts such as rabbits or mice, with validation steps including knockout cell line controls to ensure specificity. Researchers employ TLN1 antibodies to explore mechanisms underlying tumor invasion, platelet activation, and tissue development. Recent studies also highlight its role in modulating immune synapse formation in T-cells, expanding its relevance in immunology. Commercial TLN1 antibodies vary in clonality (monoclonal/polyclonal) and epitope targeting, necessitating careful selection based on experimental needs.
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