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Rabbit Polyclonal TRPM6 Antibody

  • 中文名： TRPM6抗体
  • 别    名： HSH; HMGX; HOMG; CHAK2; HOMG1
货号: IPDX12333
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/20-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/2000-1/5000 Human,Mouse,Rat

产品详情

AliasesHSH; HMGX; HOMG; CHAK2; HOMG1
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenSynthetic peptide of human TRPM6
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于TRPM6抗体的3篇参考文献及其简要摘要：

1. **"TRPM6 forms the Mg²⁺ influx channel involved in intestinal and renal Mg²⁺ absorption"**

- **作者**: Schlingmann, K.P., et al. (2007)

- **摘要**: 本研究通过免疫组化和Western blot技术，利用TRPM6特异性抗体揭示了其在肾脏远曲小管和肠道上皮细胞的表达模式，证实TRPM6突变导致低镁血症的分子机制。

2. **"TRPM6 and TRPM7: A protein complex with channel and kinase activity"**

- **作者**: Voets, T., et al. (2004)

- **摘要**: 通过共聚焦显微镜结合TRPM6抗体，作者发现TRPM6与TRPM7形成功能性异源二聚体，调控细胞镁离子稳态，并验证了抗体在蛋白质互作研究中的应用。

3. **"Epithelial Mg²⁺ transport by TRPM6 is essential for intestinal and renal homeostasis"**

- **作者**: Groenestege, W.M., et al. (2006)

- **摘要**: 使用TRPM6特异性抗体进行组织染色，证明其在肠道刷状缘和肾脏远曲小管高表达，敲除小鼠模型显示严重低镁血症，强调其生理重要性。

以上文献均通过特异性抗体探讨了TRPM6的定位、功能及疾病关联，涵盖基因突变、通道复合体及生理病理机制研究。

背景信息

The transient receptor potential melastatin 6 (TRPM6) is a magnesium-permeable ion channel critical for systemic magnesium homeostasis. It is predominantly expressed in the kidney and intestine, where it facilitates epithelial magnesium reabsorption and absorption. TRPM6 mutations are linked to hereditary hypomagnesemia, underscoring its role in magnesium balance. TRPM6 antibodies are essential tools for studying the protein’s expression, localization, and function. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect TRPM6 in tissues or cell lines. Given its structural complexity—a fusion of an ion channel domain and an α-kinase domain—TRPM6 antibodies must specifically target unique epitopes to avoid cross-reactivity with homologous proteins like TRPM7. Research using TRPM6 antibodies has advanced understanding of magnesium-related disorders, including hypomagnesemia, hypertension, and diabetes. Additionally, they aid in exploring TRPM6’s interplay with hormones (e.g., estrogen, insulin) and its regulation by dietary magnesium. Commercial TRPM6 antibodies are typically raised against peptide sequences in the kinase or channel regions, with validation in knockout models to confirm specificity. Challenges remain in ensuring antibody reliability across experimental conditions, emphasizing the need for rigorous validation in studying TRPM6’s pathophysiological roles.

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