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产品描述：马红细胞生成素(EPO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的EPO含量。EPO（促红细胞生成素）是一种糖蛋白激素，主要在肾脏产生，负责调节红细胞生成。其研究机制涉及EPO与受体结合，通过JAK2-STAT5通路促进红细胞生成，并在类风湿关节炎等疾病中发挥重要作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研领域探索EPO在生理或病理条件下的表达规律，例如研究贫血相关疾病机制、慢性肾病引起的红细胞生成异常、肿瘤微环境对造血功能的干扰，或评估药物及生物活性分子对EPO信号通路的影响。可为体外实验提供精准的定量数据支持，助力于血液学、肿瘤生物学及代谢调控等领域的分子机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EPOErythropoietin ELISA Kit
缩写：EPO
Uniprot No.：Q867B1
种属：Equus caballus (Horse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of horse EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:2 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
回收率：
| The recovery of horse EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 85-97 | |||||
| EDTA plasma (n=4) | 106 | 101-113 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.6572.5462.6022.49852.0761.9932.0351.9312.51.4001.3801.3901.2861.250.7610.8030.7820.6780.6250.4580.4340.4460.3420.3120.2950.3150.3050.2010.1560.2190.2210.2200.11600.1010.1070.104 | |||||||
货期：3-5 working days
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