首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠内皮素1(ET-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的EDN1含量。EDN1即内皮素 -1，是一种由内皮细胞合成和释放的生物活性肽。它在心血管系统等有着广泛作用，参与血管收缩、细胞增殖等生理过程。研究机制方面，聚焦其信号通路、受体相互作用，探索其在高血压、心力衰竭等疾病中的发病机制及潜在治疗靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研中ET-1表达调控机制研究，如心血管疾病动物模型的血浆ET-1水平监测、体外细胞实验中内皮细胞活化状态评估或药物干预对ET-1分泌影响的定量分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Edn1Endothelin-1 ELISA kit; ET-1 ELISA kit; Preproendothelin-1 ELISA kit; PPET1) [Cleaved into: Big endothelin-1] ELISA kit
缩写：EDN1
Uniprot No.：P22387
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 83-94 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 89-98 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-99 | |||||
回收率：
| The recovery of mouse ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-102 | ||||
| EDTA plasma (n=4) | 98 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7292.6122.6712.5245001.9381.8761.9071.7602501.2281.2041.2161.0691250.8160.7590.7880.64162.50.4350.4070.4210.27431.20.2990.2750.2870.14015.60.2410.2230.2320.08500.1510.1430.147 | ||||||
货期：3-5 working days
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