Human prothrombin fragment 1+2,F1+2 ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E09822h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人凝血酶原片段F1+2(F1+2)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的F1+2含量。人凝血酶原片段F1+2是凝血酶原激活转化为凝血酶过程中释放的稳定降解产物，其含量反映凝血酶早期激活水平，可用于诊断血栓前状态和血栓性疾病。研究显示，F1+2在凝血途径中起关键作用，其检测有助于疾病诊断和治疗监测。试剂盒检测范围为0.03 nmol/L -2 nmol/L，该产品广泛应用于基础医学研究中凝血/纤溶系统动态评估、药物抗凝效果评价、动物模型血栓形成机制探索等科研领域，为心血管疾病、肿瘤转移等病理过程的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
F1+2
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.03 nmol/L -2 nmol/L
灵敏度：
0.007 nmol/L
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Others
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-99
1:2	Average %	98
	Range %	93-102
1:4	Average %	86
	Range %	81-93
1:8	Average %	92
	Range %	88-97

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


nmol/L	OD1	OD2	Average	Corrected
2	2.296	2.274	2.285	2.137
1	1.445	1.498	1.472	1.324
0.5	0.916	0.936	0.926	0.778
0.25	0.538	0.568	0.553	0.405
0.125	0.376	0.398	0.387	0.239
0.06	0.270	0.291	0.281	0.133
0.03	0.218	0.216	0.217	0.069
0	0.146	0.149	0.148